Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta.

Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Research Abstract Details 

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Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Abstract Text:

M Hayashi,K Oshima,

Oxytocinase (cystyl-aminopeptidase) [EC 3.4.11.3] was isolated from monkey placenta in a purified form by a six-step prodedure comprising extraction from monkey placenta homogenate, ammonium sulfate fractionation, repeated chromatography on hydroxylapatite, chromatography on a column of DEAE-cellulose and gel filtration on a column of Sephadex G-200. The purified enzyme showed a single band on polyacrylamide disc electrophoresis. Oxytocin was inactivated by this enzyme preparation. The enzyme hydrolyzed several aminoacyl-beta-naphthylamides. A terminal amino group was required for enzyme activity. The molecular weight of the purified enzyme was estimated to be 87,000 by gel filtration and 83,000 by sodium dodecyl sulfate gel electrophoresis. Other properties of the enzyme, the effects of metal ions and various chemical reagents on the enzyme activity, the pH optimum, and Km values for a number of aminoacyl-beta-naphthylamides were also examined.

Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Publishing Authors By Initials

M Hayashi,K Oshima,

For similar urogenital system: genitalia: genitalia, female: uterus research abstracts see: urogenital system: genitalia: genitalia, female: uterus research

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Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 80

Page Numbers: 389-96

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1976

Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Keywords Mesh Terms:

KEYWORDS: Uterus

MESH TERMS: drug effects

Chemical & Substance for Abstract: Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta. Information

Substance Name: Cystinyl Aminopeptidase

Registry Number: EC 3.4.11.3

Grant and Affiliation Information for Purification and properties of oxytocinase (cystine amino-peptidase) from monkey placenta.

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Country: JAPAN

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MEDLINETA: J Biochem

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