Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain.

Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Research Abstract Details 

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Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Abstract Text:

M Hiraiwa,Y Uda,

Two GM1-beta-galactosidases, beta-galactosidases I, and II, have been highly purified from bovine brain by procedures including acetone and butanol treatments, and chromatographies on Con A-Sepharose, PATG-Sepharose, and Sephadex G-200. beta-Galactosidase I was purified 30,000-fold and beta-galactosidase II 19,000-fold. Both enzymes appeared to be homogeneous, as judged from the results of polyacrylamide disc gel electrophoresis. Enzyme I had a molecular weight of 600,000-700,000 and enzyme II one of 68,000, as determined on gel filtration. On sodium dodecyl sulfate polyacrylamide slab gel electrophoresis under denaturing conditions, enzyme II gave a single band with a molecular weight of 62,000, while enzyme I gave two minor bands with molecular weights of 32,000 and 20,000 in addition to the major band at 62,000. Both enzymes liberated the terminal galactose from GM1 ganglioside and lactosylceramide but not from galactosylceramide. Enzyme I showed a pH optimum of 4.0 and was heat stable, while enzyme II showed a pH optimum of 5.0 and lost 50% of its activity in 15 min at 45 degrees C. Enzyme I showed a pI of 4.2 and enzyme II one of 5.9.

Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Publishing Authors By Initials

M Hiraiwa,Y Uda,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research

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Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 707-15

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1986

Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for Purification and properties of GM1 ganglioside beta-galactosidases from bovine brain.

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Country: JAPAN

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MEDLINETA: J Biochem

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