[Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22]

[Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22] Research Abstract Details 

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[Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22] Abstract Text:

G Chen,J Du,L Zhuang,P Gao,

The five endoglucanases(CMCase) components from Aspergillus aculeatus SM-L22 were separated and purified by exclusion chromatography and ion-exchange chromatography. Five components(EG II-1, EG II-2, EG III-1, EG III-2 and EG IV) had molecular weights of 38.7, 34.4, 31.4, 36.9 and 23.7 kD by SDS-PAGE, respectively, and IEF showed their pI were pH < 3.5, < 3.5, 4.9, 4.4 and 5.0, respectively. All of them have maximum reactive activities at pH 3.5-4.0; and the optimum temperatures were 55 degrees C, 60 degrees C, 60 degrees C-70 degrees C, 60 degrees C-70 degrees C and 60 degrees C, respectively. EG II-1 and EG II-2 can only act such morphological substrates as CMC or phosphated cellulose, but EG III-1, EG III-2 and EG IV can active xylan also. The activities of all components were stimulated by Fe2+ except EG IV, EG III-2 was activited mostly by Fe2+. The kinetics' showed that there were no relativies between the affectivity of cellulases and its Km.

[Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22] Publishing Authors By Initials

G Chen,J Du,L Zhuang,P Gao,

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[Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Wei sheng wu xue bao = Acta microbiologica Sinica

VOLUME: 41

Page Numbers: 469-74

Journal Abbreviation: Wei Sheng Wu Xue Bao

ISSN: 0001-6209

DAY: 29

MONTH: Aug

YEAR: 2001

[Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22] Information

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LANGUAGE: chi

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Grant and Affiliation Information for [Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22]

AFFILIATION: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250010, China.

Country: China

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MEDLINETA: Wei Sheng Wu Xue Bao

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