Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme.

Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Research Abstract Details 

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Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Abstract Text:

K Nishio,M Kawakami,

Alanyl-tRNA synthetase was purified from the posterior silk glands of Bombyx mori by ammonium sulfate fractionation and chromatography on DEAE-Sephacel and hydroxyapatite columns. The yield was about 100 mg of the enzyme per 1 kg of the glands. The enzyme required both L-alanine and alanine tRNA for pyrophosphate formation from ATP. The PPi formation was observed even after tRNA was fully aminoacylated. The enzyme was found to be a monomer of 115K daltons by SDS-polyacrylamide gel electrophoresis, gel filtration and suberimidate cross-linking experiments. The monomeric enzyme did not dimerize in the presence of the alanine tRNA. The enzyme and the tRNA formed a 1:1 complex. The results indicate that Bombyx mori alanyl-tRNA synthetase functions in a monomeric state.

Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Publishing Authors By Initials

K Nishio,M Kawakami,

For similar amino acids, peptides, and proteins: amino acids: rna, transfer, amino acyl research abstracts see: amino acids, peptides, and proteins: amino acids: rna, transfer, amino acyl research

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Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 1867-74

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1984

Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Keywords Mesh Terms:

KEYWORDS: RNA, Transfer, Amino Acyl

MESH TERMS: metabolism

Chemical & Substance for Abstract: Purification and properties of alanyl-tRNA synthetase from Bombyx mori: a monomeric enzyme. Information

Substance Name: Alanine-tRNA Ligase

Registry Number: EC 6.1.1.7

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Country: JAPAN

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MEDLINETA: J Biochem

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