Purification and partial characterization of glutathione transferase from the teleost Monopterus albus.

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Abstract Text:

Qing Huang,Li Liang,Tao Wei,Daming Zhang,Qing-Yin Zeng,Qing Huang,Li Liang,Tao Wei,Daming Zhang,Qing-Yin Zeng,

Glutathione transferases (GSTs) catalyze the transfer of glutathione to a variety of xenobiotic and toxic endogenous compounds. GSTs are phase II biotransformation enzymes and are proposed as biomarkers of environmental pollution. In this study, a cytosolic glutathione transferase (maGST) was purified from liver of the freshwater fish Monopterus albus by affinity chromatography. The maGST appeared to be a homodimer composed of two subunits each with a molecular weight of 26 kDa. This maGST showed high activity towards the substrates 1-chloro-2,4-dinitrobenzene (CDNB) and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Kinetic analysis with CDNB as substrate revealed a K(m) of 0.28 mM and V(max) of 15.68 mumol/min per mg of protein. It had maximum activity in the pH range 7.0-7.5, a broad optimum T(m) range of 30 degrees C-55 degrees C, and a high thermal stability with 77% of its initial activity at 45 degrees C. This high thermal stability of maGST could be related to the physiological adaptation of M. albus to high temperatures in tropical and subtropical environments.

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Publishing Authors By Initials

Q Huang,L Liang,T Wei,D Zhang,QY Zeng,Q Huang,L Liang,T Wei,D Zhang,QY Zeng,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Comparative biochemistry and physiology. Toxicolog

VOLUME: 147

Page Numbers: 96-100

Journal Abbreviation: Comp. Biochem. Physiol. C Toxi

ISSN: 1532-0456

DAY: 22

MONTH: 08

YEAR: 2007

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100959500

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Purification and partial characterization of glutathione transferase from the teleost Monopterus albus.

AFFILIATION: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Graduate School, Chinese Academy of Sciences, Beijing 100049, China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Comp Biochem Physiol C Toxicol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification and partial characterization of glutathione transferase from the teleost Monopterus albus Related Publications

• BU-CTX(2) as conditioning regimen for allogeneic hematopoietic stem cell transplantation in sixty patients with leukemia.
• Effect of terazosin on blood pressure and serum lipids.
• How proteins pack into crystals: nuclei achieve translation symmetries by growing.
• Quality control of postoperative acute pain service.
• [A seroepidemiological survey: antibody to HTLV-1 in sera from various populations in Guangdong Province]
• [Application of vital dye CFDA-SE to study lymphocytic proliferation]
• [Clinical features of primary Sjgren's syndrome in childhood]
• [Clinical pathologic studies and genetic analysis of a female Duchenne muscular dystrophy family]
• [Complex mutations of 1311 C-->T in exon 11 and 93 T-->C in intron 11 in G6PD gene]
• [Correlative study between angiotensin-converting enzyme gene polymorphism and hepatorenal syndrome.]
• [DSA manifestation of primary hepatocellular carcinoma complicated by tumor thrombus in the inferior vena cava]
• [Detection of Epstein-Barr virus in T cell lymphoma and clinicopathologic analysis]
• [Determination of aceclofenac in human plasma by reversed-phase high performance liquid chromatography]
• [Determination of demethylvancomycin in neonate serum by high performance liquid chromatography]
• [Downregulation of gap junctional intercellular communication induced by silicon dioxide in the pulmonary alveolar epithelial cell]
• [Effects of microwave radiation on the content of five elements in mice bone tissue]
• [Epileptiform activity of the anterior dorsal hippocampal network induced by acute tetanization of the right posterior dorsal hippocampus of the rat]
• [Gas chromatographic analysis of methyl methacrylate and methanol in its esterification mixture]
• [Induction of human oral carcinoma by human papillomavirus 16 E6/E7 and TPA]
• [Isolation and identification of two novel polyhydroxylated sterol from Spongia obligue]
• [Localization of the connexin 43 gap-junction protein in silica-exposed alveolar epithelial cells]
• [Observation and research on the electroporation of S-180 cell induced by electric pulse]
• [Recombinant AAV-LMP-induced LMP specific cytotoxic response to autologous lymphoblastoid cell lines tranformed by Epstein-Barr virus]
• [Retinal vein occlusion with retinal arteriovenous communications]
• [Selection of optimal extraction process for magnolol by orthogonal design]
• [Serological survey of human T lymphotropic virus type I (HTLV-1) IgG antibody in south region of Xinjiang]
• [Study on occupational health hazards in building Qingzang railway and effectiveness of preventive measures.]
• [The expression of heat shock protein 70 (HSP70) in the cells of rat liver and cardiac muscle in different heat exposure stages]
• [The role of nitric oxide in hyperdynamic circulation in portal hypertensive rats]
• [Treatment of OSAS with modified twin-block advancement appliances]