Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts.

Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts. Research Abstract Details 

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Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts. Abstract Text:

Vincent Gache,Patrice Waridel,Sylvie Luche,Andrej Shevchenko,Andrei V Popov,Vincent Gache,Patrice Waridel,Sylvie Luche,Andrej Shevchenko,Andrei V Popov,

Microtubule-binding proteins are conveniently divided into two large groups: MAPs (microtubule-associated proteins), which can stabilize, anchor, and/or nucleate microtubules, and motors, which use the energy of ATP hydrolysis for a variety of functions, including microtubule network organization and cargo transportation along microtubules. Here, we describe the use of Taxol-stabilized microtubules for purification of MAPs, motors, and their complexes from Xenopus egg extracts. Isolated proteins are analysed using sodium dodecyl sulfate gel electrophoresis and identified by various mass spectrometry and database mining technologies. Found proteins can be grouped into three classes: (1) known MAPs and motors; (2) proteins previously reported as associated with the microtubule cytoskeleton, but without a clearly defined cytoskeletal function; (3) proteins not yet described as having microtubule localization. Sequence-similarity methods employed for protein identification allow efficient identification of MAPs and motors from species with yet unsequenced genomes.

Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts. Publishing Authors By Initials

V Gache,P Waridel,S Luche,A Shevchenko,AV Popov,V Gache,P Waridel,S Luche,A Shevchenko,AV Popov,

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Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in molecular medicine

VOLUME: 137

Page Numbers: 29-43

Journal Abbreviation: Methods Mol. Med.

ISSN: 1543-1894

DAY: 18

MONTH: 12

YEAR: 2007

Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts.

AFFILIATION: INSERM, Unitt 366, DRDC/CS, CEA-Grenoble, France.

Country: United States

AGENCY: United States NIGMS

GRANT: 1R01GM070986-01A1

ACRONYM: GM

MEDLINETA: Methods Mol Med

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