Purification and general properties of AMP deaminase from sheep brain.

Purification and general properties of AMP deaminase from sheep brain. Research Abstract Details 

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Purification and general properties of AMP deaminase from sheep brain. Abstract Text:

K Ito,H Yamamoto,M Mizugaki,

AMP deaminase from sheep brain was purified to homogeneity on SDS-PAGE and its general properties were investigated. The native enzyme has a molecular weight of approximately 350,000 as estimated by gel filtration and it is composed of four identical subunits with a molecular weight of 85,000 each. The purified enzyme had a specific activity of 500 units/mg protein and shows a sigmoid-shaped AMP saturation curve in the presence of 100 mM KCl. This deaminase is strongly activated by ATP and inhibited by GTP. It slightly catalyzes the hydrolysis of adenosine monosulfate (AMS), dAMP, and adenosine phosphoramidate (APA). These catalytic properties resemble those of AMP deaminase from human liver.

Purification and general properties of AMP deaminase from sheep brain. Publishing Authors By Initials

K Ito,H Yamamoto,M Mizugaki,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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Purification and general properties of AMP deaminase from sheep brain. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 259-62

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1988

Purification and general properties of AMP deaminase from sheep brain. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and general properties of AMP deaminase from sheep brain. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Purification and general properties of AMP deaminase from sheep brain. Information

Substance Name: AMP Deaminase

Registry Number: EC 3.5.4.6

Grant and Affiliation Information for Purification and general properties of AMP deaminase from sheep brain.

AFFILIATION: Department of Pharmaceutical Sciences, Tohoku University Hospital, Miyagi.

Country: JAPAN

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MEDLINETA: J Biochem

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