Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum.

Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Research Abstract Details 

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Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Abstract Text:

J H Waterborg,C M Kuyper,

Three alkaline DNases, A, B, and C, with preference for the digestion of double-stranded DNA (dsDNA) were partially purified from microplasmodia of Physarum polycephalum. They were very similar but differed in their isoelectric points. These were pH 5.8 for DNase A, 7.1 for DNase B, and 9.1 for DNase C. All three enzymes consisted of a single polypeptide chain with a molecular weight of 16,000 to 17,000, which readily formed high molecular weight complexes with low enzyme activity. These complexes could be reversibly dissociated by urea, and DNase activity was quantitatively reactivated. The DNases hydrolyzed the substrate DNA by an endonucleolytic mechanism which gave 5'-phosphorylated products. Divalent cations, MnCl2 or MgCl2, were essential for enzyme activity at the optimum pH of approximately 8.5 and at low ionic strength. The optimal conditions of pH, buffer, divalent cations and ionic strength and the extent of inhibition by salt, phosphate ions or urea differed slightly but significantly between the different isoenzymes.

Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Publishing Authors By Initials

JH Waterborg,CM Kuyper,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 651-61

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1980

Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification and enzymatic characterization of three endoDNase isoenzymes from Physarum polycephalum. Information

Substance Name: Endonucleases

Registry Number: EC 3.1.-

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Country: JAPAN

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MEDLINETA: J Biochem

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