Purification and characterization of two isoforms of T-kininogens from rat liver microsomes.

Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Research Abstract Details 

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Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Abstract Text:

K Enjyoji,H Kato,

T-Kininogen is one of the acute phase proteins, and is a precursor of T-kinin and a cysteine protease inhibitor. Two homologous T-kininogens (TI- and TII-kininogens) were isolated from microsomal fraction of inflamed rat liver, by chromatographies on columns of DEAE-Sepharose CL-6B and DEAE-5PW and by affinity chromatography on a column of anti T-kininogen monoclonal antibody. The amino terminal amino acid sequences of the two microsomal pyridylethylated T-kininogens after pyroglutamyl aminopeptidase treatment were identical with those of TI- and TII-kininogens from inflamed rat plasma. Microsomal T-kininogens moved faster on SDS-PAGE after treatment with endoglycosidase H. The amounts of microsomal TI- and TII-kininogens in inflamed and non-inflamed rat liver were quantitated by immunoblotting of homogenates of liver microsomes using anti T-kininogen rabbit antiserum. The amounts of microsomal T-kininogens were increased in inflamed rat liver, but the ratio of the amounts of TI-kininogen to TII-kininogen was not different in the inflamed and non-inflamed rat liver. On the other hand, TII-kininogen was not significantly detected in non-inflamed rat plasma. These results indicate that the secretion of one of the T-kininogens, TII-kininogen, into plasma may be prevented by some unknown mechanism.

Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Publishing Authors By Initials

K Enjyoji,H Kato,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

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Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 111

Page Numbers: 670-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1992

Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Strains

MESH TERMS: chemistry

Chemical & Substance for Abstract: Purification and characterization of two isoforms of T-kininogens from rat liver microsomes. Information

Substance Name: Kininogens

Registry Number: 0

Grant and Affiliation Information for Purification and characterization of two isoforms of T-kininogens from rat liver microsomes.

AFFILIATION: National Cardiovascular Center Research Institute, Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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