Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica).

Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica). Research Abstract Details 

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Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica). Abstract Text:

Zhiqiang Wu,Guoliang Jiang,Peng Xiang,Dong Yang,Ning Wang,

Two trypsin-like enzymes (TLEs) were purified from North Pacific krill (Euphausia pacifica) by ammonium sulfate precipitation, ion-exchange and gel-filtration chromatography. The purified enzymes were identified as trypsins by LC-ESI-MS/MS analysis. The relative molecular mass of TLE I and TLE II were 33 and 32.3 kDa, respectively, with isoelectric points of 4.5 and 4.3, respectively. The TLEs showed excellent thermal stable in the crude extract and the purified TLEs were active over a wide pH (6.0-11.0) and temperature (10-70 degrees C) range. Compared with trypsins from other organisms, the purified TLEs had physiological efficiencies of 1.6-6.7-fold. The difference in Arg, Ile and Asp content might explain why E. pacifica TLEs have good thermal stability and physiological efficiency.

Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica). Publishing Authors By Initials

Z Wu,G Jiang,P Xiang,D Yang,N Wang,

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Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology letters

VOLUME: 30

Page Numbers: 67-72

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 7

MONTH: 11

YEAR: 2007

Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica). Information

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LANGUAGE: eng

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Grant and Affiliation Information for Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica).

AFFILIATION: College of Marine Life Science, Ocean University of China, Qingdao, 266003, China, gljiang@ouc.edu.cn.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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