Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus.

Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus. Research Abstract Details 

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Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus. Abstract Text:

Yumi Oba,Yohei Shimasaki,Yuji Oshima,Hina Satone,Takeshi Kitano,Miki Nakao,Shun-ichiro Kawabata,Tsuneo Honjo,Yumi Oba,Yohei Shimasaki,Yuji Oshima,Hina Satone,Takeshi Kitano,Miki Nakao,Shun-ichiro Kawabata,Tsuneo Honjo,

We used gel filtration chromatography, anion-exchange chromatography and polyacrylamide gel electrophoresis to purify tributyltin-binding protein type 2 (TBT-bp 2) from plasma of Japanese flounder (Paralichthys olivaceus) injected intraperitoneally with TBT (5.0 mg/kg body weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the molecular mass of TBT-bp 2 was approximately 48 kDa, and isoelectric focusing-polyacrylamide gel electrophoresis indicated that the isoelectric point was approximately 3.0. TBT-bp 2 contained 40% N-glycan. The complete cDNA nucleotide sequence and the genome sequence of TBT-bp 2 were determined by means of rapid amplification of cDNA ends of liver tissue of Japanese flounder and a genome-walking technique, respectively. The 216 amino acid sequence of TBT-bp 2 showed 47% identity to the sequences of puffer fish (Takifugu pardalis) saxitoxin- and tetrodotoxin-binding protein but only 27% similarity to the sequence of TBT-bp 1. Analysis of the motif sequence of the amino acid sequence and the structure of the gene encoding TBT-bp 2 suggested that this protein belongs to the lipocalin superfamily.

Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus. Publishing Authors By Initials

Y Oba,Y Shimasaki,Y Oshima,H Satone,T Kitano,M Nakao,S Kawabata,T Honjo,Y Oba,Y Shimasaki,Y Oshima,H Satone,T Kitano,M Nakao,S Kawabata,T Honjo,

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Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 142

Page Numbers: 229-38

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 1

MONTH: 06

YEAR: 2007

Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus. Information

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LANGUAGE: eng

NlmUniqueID: 376600

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Grant and Affiliation Information for Purification and characterization of tributyltin-binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus.

AFFILIATION: Laboratory of Marine Environmental Science, Division of Marine Biological Chemistry, Department of Bioscience and Biotechnology, Kyushu University, Higashi-ku, Fukuoka, 812-8581, Japan.

Country: Japan

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MEDLINETA: J Biochem (Tokyo)

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