Purification and characterization of soluble human IL-6 receptor expressed in CHO cells.

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Research Abstract Details 

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Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Abstract Text:

K Yasukawa,T Saito,T Fukunaga,Y Sekimori,Y Koishihara,H Fukui,Y Ohsugi,T Matsuda,H Yawata,T Hirano,

An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensitivity of M1 cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml.

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Publishing Authors By Initials

K Yasukawa,T Saito,T Fukunaga,Y Sekimori,Y Koishihara,H Fukui,Y Ohsugi,T Matsuda,H Yawata,T Hirano,

For similar natural sciences: chemistry: chemistry, physical: solubility research abstracts see: natural sciences: chemistry: chemistry, physical: solubility research

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Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 673-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1990

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Keywords Mesh Terms:

KEYWORDS: Solubility

MESH TERMS: genetics

Chemical & Substance for Abstract: Purification and characterization of soluble human IL-6 receptor expressed in CHO cells. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Purification and characterization of soluble human IL-6 receptor expressed in CHO cells.

AFFILIATION: Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa.

Country: JAPAN

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MEDLINETA: J Biochem

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