Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster.

Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Research Abstract Details 

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Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Abstract Text:

K Fujimoto,K Masuda,N Asada,E Ohnishi,

Two isoforms of prophenoloxidase were isolated from pupae of Oregon-R strain of Drosophila melanogaster. The purification procedure included ammonium sulfate fractionation, Sephacryl S-200 gel chromatography, DEAE-cellulose, and hydroxylapatite column chromatography. The two isoforms, A1 and A3, could be separated by ammonium sulfate fractionation. The isoelectric points of A1 and A3 were determined to be pH 5.8 and 6.7, respectively. The molecular weights of the monomers of A1 and A3 were estimated by SDS-PAGE to be 78 and 77 kDa, respectively. The native states of A1 and A3 are considered to be homodimeric, as judged by gel-filtration chromatography.

Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Publishing Authors By Initials

K Fujimoto,K Masuda,N Asada,E Ohnishi,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 285-91

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1993

Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. Information

Substance Name: Catechol Oxidase

Registry Number: EC 1.10.3.1

Grant and Affiliation Information for Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster.

AFFILIATION: Biological Laboratory, Faculty of Science, Okayama University of Science.

Country: JAPAN

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MEDLINETA: J Biochem

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