Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis.

Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis. Research Abstract Details 

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Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis. Abstract Text:

Fan Li,Sha Wang,Weifeng Liu,Guanjun Chen,

Polylactide or poly(l-lactic acid) (PLA) is a commercially promising material for use as a renewable and biodegradable plastic. Three novel PLA-degrading enzymes, named PLAase I, II and III, were purified to homogeneity from the culture supernatant of an effective PLA-degrading bacterium, Amycolatopsis orientalis ssp. orientalis. The molecular masses of these three PLAases as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were 24.0, 19.5 and 18.0 kDa, with the pH optima being 9.5, 10.5 and 9.5, respectively. The optimal temperature for the enzyme activities was 50-60 degrees C. All the purified enzymes could degrade high-molecular-weight PLA film as well as casein, and the PLA-degrading activities were strongly inhibited by serine protease inhibitors such as phenylmethylsulfonyl fluoride and aprotinin, but were not susceptive to chymostatin and pepstatin. Taken together, these data demonstrated that A. orientalis ssp. orientalis produces multiple serine-like proteases to utilize extracellular polylactide as a sole carbon source.

Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis. Publishing Authors By Initials

F Li,S Wang,W Liu,G Chen,

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Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: FEMS microbiology letters

VOLUME: 282

Page Numbers: 52-8

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 18

MONTH: 03

YEAR: 2008

Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Purification and characterization of poly(l-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis.

AFFILIATION: The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, Shandong, China.

Country: England

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MEDLINETA: FEMS Microbiol Lett

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