Purification and characterization of membrane-bound phospholipase A2 from rat platelets.

Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Research Abstract Details 

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Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Abstract Text:

M Hayakawa,I Kudo,M Tomita,K Inoue,

Phospholipase A2 was solubilized from rat platelet membrane by 1 M KCl and purified to near homogeneity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and HPLC. The characteristics of the purified membrane-bound enzyme were compared with those of phospholipase A2 released from thrombin-stimulated rat platelets (Horigome, K., Hayakawa, M., Inoue, K., & Nojima, S. (1987) J. Biochem. 101, 625-631). The molecular weights, elution profiles on reversed-phase HPLC, and NH2-terminal sequences were identical for the two enzymes. Other characteristics of the two enzymes, such as specific activity, substrate specificity, pH optimum, Ca2+ requirement, heat lability, and sensitivity to p-bromophenacyl bromide were also indistinguishable. These findings suggest that both enzymes share a common structure.

Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Publishing Authors By Initials

M Hayakawa,I Kudo,M Tomita,K Inoue,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: thrombin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: thrombin research

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Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 263-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1988

Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Keywords Mesh Terms:

KEYWORDS: Thrombin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification and characterization of membrane-bound phospholipase A2 from rat platelets. Information

Substance Name: Thrombin

Registry Number: EC 3.4.21.5

Grant and Affiliation Information for Purification and characterization of membrane-bound phospholipase A2 from rat platelets.

AFFILIATION: Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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