Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis.

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Abstract Text:

S Hara,I Kudo,H W Chang,K Matsuta,T Miyamoto,K Inoue,

Extracellular phospholipase A2 was purified about 1.7 X 10(5) fold to near homogeneity from human synovial fluid of rheumatoid arthritis by sequential use of column chromatographies on heparin-Sepharose, butyl-Toyopearl, and reversed-phase HPLC. The final preparation showed a single band on SDS-polyacrylamide gel electrophoresis, and its molecular mass was estimated to be approximately 13,700 daltons. The purified enzyme had a pH optimum of 9.0 and required Ca2+ for maximum activity. It hydrolyzed phosphatidyl-ethanolamine more effectively than phosphatidylserine and phosphatidylcholine. These properties were similar to those of an extracellular phospholipase A2 detected in the peritoneal cavity of caseinate-treated rats.

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Publishing Authors By Initials

S Hara,I Kudo,HW Chang,K Matsuta,T Miyamoto,K Inoue,

For similar musculoskeletal system: skeleton: joints: joint capsule: synovial membrane: synovial fluid research abstracts see: musculoskeletal system: skeleton: joints: joint capsule: synovial membrane: synovial fluid research

PUBMED ID PMID:

MEDLINE DATE:

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 105

Page Numbers: 395-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1989

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Keywords Mesh Terms:

KEYWORDS: Synovial Fluid

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis. Information

Substance Name: Phospholipases A2

Registry Number: EC 3.1.1.4

Grant and Affiliation Information for Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis.

AFFILIATION: Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis Related Publications

• A 'reverse' phylogenetic approach for identification of novel osmoregulatory and cardiovascular hormones in vertebrates.
• Determination of microscopic ionization constants and microscopic stacking equilibrium quotients of adenylyl(3' --> 5')adenosine.
• Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair.
• Effect of French maritime pine bark extract on endometriosis as compared with leuprorelin acetate.
• Expression of Dmp1 in specific differentiated, nonproliferating cells and its regulation by E2Fs.
• Factors Contributing to Cavitation after CT-guided Percutaneous Radiofrequency Ablation for Lung Tumors.
• Hardness and elasticity of sound and caries-affected primary dentin bonded with one-step self-etch adhesive.
• Increased expression of ephrin A1 in brain arteriovenous malformation: DNA microarray analysis.
• Influence of playing wind instruments on activity of masticatory muscles.
• Inter-observer variations in FDG-PET interpretation for cancer screening.
• Intervertebral disc degeneration: biological and biomechanical factors.
• Lack of mitochondrial DNA enhances growth of hepatocellular carcinoma in vitro and in vivo.
• Mannose-conjugated alendronate selectively depletes Kupffer cells and inhibits endotoxemic shock in the mice.
• Mechanical properties of comonomer-compositionally fractionated poly[(3-hydroxybutyrate)-co-(3-mercaptopropionate)] with low 3-mercaptopropionate unit content.
• Microcirculation in eyes after rhegmatogenous retinal detachment surgery.
• Microembolic signals are associated with progression of arterial lesion in Moyamoya disease: a case report.
• Neurosprora crassa RAD5 homologue, mus-41, inactivation results in higher sensitivity to mutagens but has little effect on PCNA-ubiquitylation in response to UV-irradiation.
• Novel correction methods as alternatives for the six-dimensional correction in CyberKnife treatment.
• Organelles containing inositol trisphosphate receptor type 2 in adrenal medullary cells.
• Pharmacological cardioversion of long-lasting atrial fibrillation.
• Radiological follow-up study of rheumatoid wrists after radio-lunate limited arthrodesis with ulnar head resection.
• Relation of interleukin-6 and C-reactive protein levels to sinus maintenance after pharmacological cardioversion in persistent atrial fibrillation.
• Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis.
• Spin transition at the mesophase transition temperature in a cobalt(II) compound with branched alkyl chains.
• Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway.
• Thioredoxin overexpression in mice, model of attenuation of oxidative stress, prevents benzene-induced hemato-lymphoid toxicity and thymic lymphoma.
• Tumour necrosis factor alpha-stimulated gene-6 inhibits osteoblastic differentiation of human mesenchymal stem cells induced by osteogenic differentiation medium and BMP-2.
• Visual reflex seizures induced by complex stimuli.
• [Current and future prospects for anti-osteoporotic drugs]
• [Syntheses and spectra of Mn(III)-chlorophyll-a and Mn(II)-chlorophyll-a]