Purification and characterization of Escherichia coli DNA polymerase V.

Purification and characterization of Escherichia coli DNA polymerase V. Research Abstract Details 

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Purification and characterization of Escherichia coli DNA polymerase V. Abstract Text:

Katharina Schlacher,Qingfei Jiang,Roger Woodgate,Myron F Goodman,

Cell survival and genome rescue after UV irradiation in Escherichia coli depends on DNA repair mechanisms induced in response to DNA damage as part of the SOS regulon. SOS occurs in two phases. The first phase is dominated by accurate repair processes such as excision and recombinational DNA repair, while the second phase is characterized by a large approximately 100-fold increase in mutations caused by an error-prone replication of damaged DNA templates. SOS mutagenesis occurs as a direct result of the action of the UmuDC gene-products, which form the low fidelity Escherichia coli DNA polymerase V, a heterotrimeric complex composed of UmuD'(2)C. This chapter describes the preparation of highly purified native pol V that is suitable for a wide range of biochemical studies of protein-protein, protein-DNA interactions and translesion-synthesis (TLS) mechanisms.

Purification and characterization of Escherichia coli DNA polymerase V. Publishing Authors By Initials

K Schlacher,Q Jiang,R Woodgate,MF Goodman,

For similar proteins: bacterial proteins: escherichia coli proteins research abstracts see: proteins: bacterial proteins: escherichia coli proteins research

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Purification and characterization of Escherichia coli DNA polymerase V. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Methods in enzymology

VOLUME: 408

Page Numbers: 378-90

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 3

MONTH: 12

YEAR: 2006

Purification and characterization of Escherichia coli DNA polymerase V. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 212271

Purification and characterization of Escherichia coli DNA polymerase V. Keywords Mesh Terms:

KEYWORDS: Escherichia coli Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Purification and characterization of Escherichia coli DNA polymerase V. Information

Substance Name: DNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.7

Grant and Affiliation Information for Purification and characterization of Escherichia coli DNA polymerase V.

AFFILIATION: Department of Biological Sciences, University of Southern California, Los Angeles, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R37GM21422

ACRONYM: GM

MEDLINETA: Methods Enzymol

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