Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight.

Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Research Abstract Details 

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Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Abstract Text:

S Yasugi,T Mizuno,

An embryo-specific pepsinogen was isolated from the proventriculi of 15-day-old chicken embryos and purified by means of fractionation with ammonium sulfate, filtration on Sephadex G-100, and chromatography on DEAE-Sepharose CL-6B and hydroxyapatite. The properties of this pepsinogen and pepsin derived from it were compared with those of an adult-specific chicken pepsinogen and its pepsin. Though the optimal pH and alkali-stability were similar in the two pepsinogens, molecular weight, sensitivity to pepstatin, and antigenicity were quite different. Among the properties of this embryo-specific pepsinogen, the large molecular weight (56,000 for pepsinogen and 53,000 for pepsin) is especially noteworthy, since the molecular weights of the known pepsinogens of mammals and birds fall into the range of 35,000-48,000.

Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Publishing Authors By Initials

S Yasugi,T Mizuno,

For similar peptides: oligopeptides: pepstatins research abstracts see: peptides: oligopeptides: pepstatins research

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Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 89

Page Numbers: 311-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1981

Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Keywords Mesh Terms:

KEYWORDS: Pepstatins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight. Information

Substance Name: Pepsin A

Registry Number: EC 3.4.23.1

Grant and Affiliation Information for Purification and characterization of embryonic chicken pepsinogen, a unique pepsinogen with large molecular weight.

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Country: JAPAN

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MEDLINETA: J Biochem

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