Purification and characterization of diamine oxidase (histaminase) from rat small intestine.

Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Research Abstract Details 

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Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Abstract Text:

H Mizuguchi,I Imamura,M Takemura,H Fukui,

Diamine oxidase (DAO) was purified to homogeneity from rat small intestine, and its biochemical and immunochemical properties were studied. DAO was suggested to be a dimer of a 92 kDa subunit, and its isoelectric point was found to be 6.0. Histamine, putrescine, N tau-methylhistamine, and cadaverine were good substrates, with Km values ranging from 9.4 to 16.0 microM. Spermine and spermidine were not substrates. Both an immunoprecipitation study and Ouchterlony's double diffusion test involving antiserum against the purified DAO showed that the immunological properties of the DAOs from rat small intestine, thymus, and placenta were identical. Among small intestinal DAOs from different species, this antibody reacted to the guinea pig enzyme as strongly as to the rat enzyme, but the reaction was much weaker to the mouse enzyme than to the rat enzyme. The DAOs from rabbit and dog small intestine, pig kidney, and human placenta showed no reactivity toward this antibody.

Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Publishing Authors By Initials

H Mizuguchi,I Imamura,M Takemura,H Fukui,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 631-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1994

Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: physiology

Chemical & Substance for Abstract: Purification and characterization of diamine oxidase (histaminase) from rat small intestine. Information

Substance Name: Amine Oxidase (Copper-Containing)

Registry Number: EC 1.4.3.6

Grant and Affiliation Information for Purification and characterization of diamine oxidase (histaminase) from rat small intestine.

AFFILIATION: Department of Pharmacology II, Faculty of Medicine, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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