Purification and characterization of chitin deacetylase from Absidia coerulea.

Purification and characterization of chitin deacetylase from Absidia coerulea. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification and characterization of chitin deacetylase from Absidia coerulea. Abstract Text:

X D Gao,T Katsumoto,K Onodera,

Chitin deacetylase, which releases the acetyl groups of glycol chitin was purified from a fungus, Absidia coerulea, and characterized. The enzyme was purified 516-fold to homogeneity by means of 65-80% ammonium sulfate precipitation followed by chromatography on Butyl Toyoperal-650M, Gigapite (hydroxyapatite), and DEAE Toyopearl-650M. It had an apparent molecular weight of 75 kDa both on sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration chromatography, indicating that the enzyme exists as a monomer. The amino-terminal sequence was determined to be Gly-Glu-Tyr-Trp-Gln-Ser-Phe-. The enzyme is active on chitooligosaccharides with more than two N-acetylglucosamine residues (chitobiose) and is able to convert the nascent chitin synthesized by chitin synthase to chitosan in vitro. When O-hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pH for enzyme activity was 5.0 and the optimum temperature was 50 degrees C. The enzyme was heat-stable and strongly inhibited by Fe3+. Furthermore, chitin deacetylase was demonstrated to be localized near the inner face of the cell wall (periplasmic space) in the mycelia by using immunoelectron microscopy.

Purification and characterization of chitin deacetylase from Absidia coerulea. Publishing Authors By Initials

XD Gao,T Katsumoto,K Onodera,

For similar fungi: zygomycota research abstracts see: fungi: zygomycota research

PUBMED ID PMID:

MEDLINE DATE:

Purification and characterization of chitin deacetylase from Absidia coerulea. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 117

Page Numbers: 257-63

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1995

Purification and characterization of chitin deacetylase from Absidia coerulea. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of chitin deacetylase from Absidia coerulea. Keywords Mesh Terms:

KEYWORDS: Zygomycota

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and characterization of chitin deacetylase from Absidia coerulea. Information

Substance Name: chitin deacetylase

Registry Number: EC 3.5.1.41

Grant and Affiliation Information for Purification and characterization of chitin deacetylase from Absidia coerulea.

AFFILIATION: Division of Agriculture and Agricultural Life Sciences, University of Tokyo.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification and characterization of chitin deacetylase from Absidia coerulea Related Publications

• A monoclonal antibody (1D12) defines novel distribution patterns of prion protein (PrP) as granules in nucleus.
• A new technique to achieve sufficient mesorectal excision in upper rectal cancer.
• Active stabilization of a higher-order mode-locked fiber laser operating at a pulse-repetition rate of 154 GHz.
• An immunotherapy approach with dendritic cells genetically modified to express the tumor-associated antigen, HER2.
• Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament.
• Clinical, molecular and histopathological features of short stature syndrome with novel CUL7 mutation in Yakuts: new population isolate in Asia.
• Deterioration of urinary findings after tonsil stimulation in patients with IgA nephropathy.
• Development of efficient method for purified recombinant bovine granulocyte-macrophage colony-stimulating factor production with baculovirus-silkworm gene expression system.
• Distinct immunohistochemical localization in Kuru plaques using novel anti-prion protein antibodies.
• Effect of actinomycin and mitomycin on fibroin synthesis in the posterior silkgland of Bombyx mori.
• Fourier synthesis of 1.8-THz optical-pulse trains by phase locking of three independent semiconductor lasers.
• Heterogeneity of a human erythrocyte membrane glycoprotein.
• Incorporation of glycine-C14 into fibroin in subcellular fractions of the posterior silkgland of the silkworm, Bombyx mori, L.
• Intracranial vertebral artery dissection in Wallenberg syndrome.
• Lensed-taper launching coupler for small-bore, infrared hollow fibers.
• Monitoring of cholinesterase-inhibiting activity in water from the Tone canal, Japan, as a biomarker of ecotoxicity.
• Monitoring of triclosan in the surface water of the tone canal, Japan.
• Neurotoxic prion protein (PrP) fragment 106-126 requires the N-terminal half of the hydrophobic region of PrP in the PrP-deficient neuronal cell line.
• Purification and characterization of chitin deacetylase from Absidia coerulea.
• Reversible aggregation of a human erythrocyte membrane glycoprotein.
• Role of renomedullary thromboxane A2 in development of doca-salt hypertension.
• Serum withdrawal-induced apoptosis in ZrchI prion protein (PrP) gene-deficient neuronal cell line is suppressed by PrP, independent of Doppel.
• Soluble polyglutamine oligomers formed prior to inclusion body formation are cytotoxic.
• Stable transgene expression in mice generated from retrovirally transduced embryonic stem cells.
• TDP-43 mutation in familial amyotrophic lateral sclerosis.
• The relationship between 1-deoxynojirimycin content and alpha-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) in Kyoto, Japan.
• [Current health problems and innovations to be made--factors which promote or inhibit the changes]
• [Gap between patients' needs and nursing actions]
• [Nursing and self care: definition of self care]
• [Physical and personnel requirements in clinical training - problems in nursing administration]