Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma).

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Abstract Text:

T Niidome,N Yoshida,F Ogata,A Ito,K Noda,

An acidic amino acid-specific endopeptidase was purified from Protease Type XVI (Sigma), a commercial product from culture filtrate of Bacillus subtilis, by a series of column chromatographies on CM-Toyopearl (Fractogel) and Mono-S, guided by activity assay using Boc-Ala-Ala-Pro-Glu-pNA as a substrate. The final preparation was homogeneous on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase HPLC. The molecular weight of the protease was estimated to be 18,000 by gel filtration on TSK gel G3000SWXL column using 6 M guanidine hydrochloride as an eluent, and 17,000 by SDS-PAGE in the presence of 2-mercaptoethanol. The isoelectric point of the protease was 7.7. Studies on the substrate specificity with peptide p-nitroanilides and natural peptides revealed that the protease hydrolyzes the peptide bonds on the carboxyl-terminal side of acidic amino acids, especially of glutamic acid. The protease was completely inactivated by DFP, indicating the serine protease nature of the protease. The activity of the protease was also inhibited by EDTA and GEDTA, and reactivated by Ca2+. The protease contained 1.3 +/- 0.2 mol/mol protein of Ca2+. These results suggest that Ca2+ plays a vital role in the protease activity.

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Publishing Authors By Initials

T Niidome,N Yoshida,F Ogata,A Ito,K Noda,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

PUBMED ID PMID:

MEDLINE DATE:

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 108

Page Numbers: 965-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1990

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma). Information

Substance Name: Endopeptidases

Registry Number: EC 3.4.-

Grant and Affiliation Information for Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation (Protease Type XVI, Sigma).

AFFILIATION: Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification and characterization of an acidic amino acid-specific endopeptidase of Bacillus subtilis obtained from a commercial preparation Protease Type XVI, Sigma Related Publications

• A study on the mechanism of superprecipitation of myosin B.
• An osteogenesis-related transcription factor, core-binding factor A1, is constitutively expressed in the chondrocytic cell line TC6, and its expression is upregulated by bone morphogenetic protein-2.
• Antibody for collagenase from Clostridium histolyticum.
• Bone morphogenetic protein regulation of forkhead/winged helix transcription factor Foxc2 (Mfh1) in a murine mesodermal cell line C1 and in skeletal precursor cells.
• Bone morphogenetic protein-2 enhances osterix gene expression in chondrocytes.
• Calibration-curve-free quantitative PCR: a quantitative method for specific nucleic acid sequences without using calibration curves.
• Compositions of royal jelly II. Organic acid glycosides and sterols of the royal jelly of honeybees (Apis mellifera).
• Coordinated expression of noggin and bone morphogenetic proteins (BMPs) during early skeletogenesis and induction of noggin expression by BMP-7.
• Desulfation of L-serine-O-sulfate and L-threonine-O-sulfate by human plasma.
• Dynamic control of the Q factor in a photonic crystal nanocavity.
• Effects of phosphorus contents on the gelatinization and retrogradation of potato starch.
• Extension of quadrature orthogonal signal corrected two-dimensional (QOSC 2D) correlation spectroscopy I: principal component analysis based QOSC 2D.
• Germ line and somatic mutations of BRAF V599E in ovarian carcinoma.
• Guide oligonucleotide-dependent DNA linkage that facilitates controllable polymerization of microgene blocks.
• Identification of DERMO-1 as a member of helix-loop-helix type transcription factors expressed in osteoblastic cells.
• Inhibitory helix-loop-helix transcription factors Id1/Id3 promote bone formation in vivo.
• Intracellular transport of phosphatidic acid and phosphatidylcholine into lipid bodies in an oleaginous fungus, Mortierella ramanniana var. angulispora.
• Metabolic effect of infused amino acid solutions during anesthesia and surgical stress.
• Molecular organization of cell membranes isolated from myxamoebae of cellular slime mold, Dictyostelium discoideum. I. Presence of membrane subunits and the physical properties.
• Molecular organization of cell membranes isolated from myxamoebae of cellular slime mold, Dictyostelium discoideum. II. Reassociation of membrane subunits.
• Negative regulation of bone morphogenetic protein/Smad signaling by Cas-interacting zinc finger protein in osteoblasts.
• Operational performance of the NIJI-III superconducting storage ring.
• Palliative care needs of cancer outpatients receiving chemotherapy: an audit of a clinical screening project.
• PlexinD1 deficiency induces defects in axial skeletal morphogenesis.
• Scleraxis messenger ribonucleic acid is expressed in C2C12 myoblasts and its level is down-regulated by bone morphogenetic protein-2 (BMP2).
• Spaciotemporal association and bone morphogenetic protein regulation of sclerostin and osterix expression during embryonic osteogenesis.
• The nucleocytoplasmic shuttling protein CIZ reduces adult bone mass by inhibiting bone morphogenetic protein-induced bone formation.
• The serine racemase mRNA is predominantly expressed in rat brain neurons.
• Transcriptional expression of survivin and its splice variants in cervical carcinomas.
• Tyrosine phosphorylation of ErbB4 is enhanced by PSD95 and repressed by protein tyrosine phosphatase receptor type Z.