Purification and characterization of alkaline phosphatase in cultured rat liver cells.

Purification and characterization of alkaline phosphatase in cultured rat liver cells. Research Abstract Details 

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Purification and characterization of alkaline phosphatase in cultured rat liver cells. Abstract Text:

Y Yokota,

Alkaline phosphatase has been purified from cultured rat liver cells by butanol extraction, column chromatography on DEAE-cellulose and on Sephadex G-200, and preparative polyacrylamide gel electrophoresis. By electrophoresis on polyacrylamide, the purified enzyme was resolved into two active forms. Both forms have similar molecular weights of around 200,000. The subunit size was found to be 50,000 by SDS-polyacrylamide gel electrophoresis. These results suggest that alkaline phosphatase purified from cultured rat liver cells has a tetrameric structure. The optimum pH was found to be approximately 10.4, using p-nitrophenylphosphate as a substrate in a carbonate buffer system. The apparent Km was estimated to be 2.4 mM, using p-nitrophenylphosphate in carbonate buffer, pH 10.4.

Purification and characterization of alkaline phosphatase in cultured rat liver cells. Publishing Authors By Initials

Y Yokota,

For similar natural sciences: chemistry: chemistry, physical: molecular weight research abstracts see: natural sciences: chemistry: chemistry, physical: molecular weight research

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Purification and characterization of alkaline phosphatase in cultured rat liver cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 83

Page Numbers: 1285-92

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1978

Purification and characterization of alkaline phosphatase in cultured rat liver cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of alkaline phosphatase in cultured rat liver cells. Keywords Mesh Terms:

KEYWORDS: Molecular Weight

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and characterization of alkaline phosphatase in cultured rat liver cells. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

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Country: JAPAN

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MEDLINETA: J Biochem

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