Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells.

Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Research Abstract Details 

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Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Abstract Text:

Y Yokota,

Alkaline phosphatase of cultured rat ascites hepatoma cells has been purified by butanol extraction, DEAE-cellulose column chromatography, gel filtration through Sephadex G-200, concanavalin A-Sepharose affinity chromatography, and polyacrylamide gel electrophoresis. Affinity chromatography confirmed the glycoprotein nature of alkaline phosphatase from cultured rat ascites hepatoma cells. Electrophoresis on polyacrylamide gels of various concentrations indicated a molecular weight of 290,000. The molecular weight of the subunit was estimated to be 72,000 by SDS-polyacrylamide gel electrophoresis. These findings suggest that alkaline phosphatase of cultured rat ascites hepatoma cells is a tetramer with a subunit molecular weight of 72,000.

Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Publishing Authors By Initials

Y Yokota,

For similar neoplasms: neoplasms, experimental research abstracts see: neoplasms: neoplasms, experimental research

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Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 83

Page Numbers: 1293-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1978

Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Keywords Mesh Terms:

KEYWORDS: Neoplasms, Experimental

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and characterization of alkaline phosphatase from cultured rat ascites hepatoma cells. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

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Country: JAPAN

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MEDLINETA: J Biochem

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