Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli.

Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Research Abstract Details 

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Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Abstract Text:

T Suzuki,S Ichihara,S Mizushima,

A signal peptide, a processing product of the precursor of the lipoprotein in the cytoplasmic membrane of Escherichia coli, has been purified through extractions with butanol and ethyl ether and chromatographies with a Sephadex LH-60 column and Sep-pak C18. Analysis of the amino acid composition and sequencing of the N- and C-termini indicate that the signal peptide was intact, suggesting that the first step of the signal peptide catabolism in the cytoplasmic membrane is the cleavage of the intact signal peptide. During the purification, the signal peptide exhibited unique features, including strong interaction with phospholipids. The possible importance of such features in the process of protein translocation across membranes is discussed.

Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Publishing Authors By Initials

T Suzuki,S Ichihara,S Mizushima,

For similar peptides: protein sorting signals research abstracts see: peptides: protein sorting signals research

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Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 470-3

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1988

Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Protein Sorting Signals

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli. Information

Substance Name: Protein Sorting Signals

Registry Number: 0

Grant and Affiliation Information for Purification and characterization of a signal peptide, a product of protein secretion across the cytoplasmic membrane of Escherichia coli.

AFFILIATION: Laboratory of Microbiology, School of Agriculture, Nagoya University, Aichi.

Country: JAPAN

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MEDLINETA: J Biochem

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