Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium.

Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Research Abstract Details 

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Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Abstract Text:

M Kojima,K Mizuno,K Kangawa,H Matsuo,

In many peptide hormones and neuropeptides, the carboxy-terminal alpha-amide structure is essential in eliciting biological activity. Here we report the purification and characterization of an alpha-amidating enzyme from porcine atrium, in which a high concentration of alpha-amidating activity was detected. The enzyme was purified to homogeneity from the membrane fraction of porcine atria by five steps of chromatography, including an affinity chromatography using a Sepharose column coupled with a substrate, Tyr-Phe-Gly. The purified enzyme was found to be composed of a single polypeptide chain with an apparent molecular weight of 92,000. This enzyme converted several synthetic peptides with C-terminal glycine to the corresponding des-glycine peptide alpha-amides.

Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Publishing Authors By Initials

M Kojima,K Mizuno,K Kangawa,H Matsuo,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 105

Page Numbers: 440-3

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1989

Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium. Information

Substance Name: Oxidoreductases Acting on CH-NH Group Do

Registry Number: EC 1.5.-

Grant and Affiliation Information for Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium.

AFFILIATION: Department of Biochemistry, Miyazaki Medical College.

Country: JAPAN

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MEDLINETA: J Biochem

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