Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta.

Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Research Abstract Details 

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Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Abstract Text:

M Nojima,S Ishiura,T Yamamoto,T Okuyama,H Furuya,H Sugita,

We purified a high-molecular-weight protease, ingensin, from extract of human placenta by successive DEAE-cellulose, hydroxyapatite, and high performance liquid chromatographies. The activity of ingensin was determined by using a synthetic substrate, succinyl-leucyl-leucyl-valyl-tyrosine-methylcoumarinamide (MCA). The purified ingensin, which gave a single band in 6.5% nondenaturing polyacrylamide gel electrophoresis, was activated by linoleic acid and sodium dodecyl sulfate (SDS). Maximum activity was observed at pH 9.5 in the presence of 0.06% SDS, but at pH 8.0 in the presence of linoleic acid. A subcellular fractionation study showed that a large amount of ingensin activity was present in the cytosol or microsome fraction rather than in the precipitate of low-speed centrifugation. The effect of protease inhibitors on the activated ingensin was also investigated.

Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Publishing Authors By Initials

M Nojima,S Ishiura,T Yamamoto,T Okuyama,H Furuya,H Sugita,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 99

Page Numbers: 1605-11

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1986

Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta. Information

Substance Name: Proteasome Endopeptidase Complex

Registry Number: EC 3.4.25.1

Grant and Affiliation Information for Purification and characterization of a high-molecular-weight protease, ingensin, from human placenta.

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Country: JAPAN

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MEDLINETA: J Biochem

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