Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus.

Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus. Research Abstract Details 

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Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus. Abstract Text:

Haijie Yang,Tian Luo,Fang Li,Shaojing Li,Xun Xu,

A natural lectin (nominated PjLec) was isolated from haemolymph of the shrimp Penaeus japonicus by affinity chromatography with fetuin-Sepharose. The result of SDS-PAGE showed that the purified PjLec protein consisted of 37kDa subunits. The native PjLec behaved as a 452kDa protein in gel filtration chromatography. Those data suggest that PjLec is composed of 12 subunits of similar molecular weight. PjLec has a broad spectrum of bacterial-agglutination activities against both Gram-positive and Gram-negative bacteria, including two Vibrio species and two other strains pathogenic for shrimp. In addition, PjLec could agglutinate all the vertebrate erythrocytes tested, and the haemagglutination was calcium-independent. The haemagglutination of PjLec was inhibited by ManNAc, Neu5A and lipopolysaccharide. Bovine submaxillary mucin, which contains mainly Neu5A, was the most potent inhibitor of PjLec (MIC of 0.0006mgml(-1)). The haemagglutination activity of PjLec was stable between pH 6 and pH 8, and was temperature-dependent. Our results suggested that PjLec may be an important humoral defence factor against bacterial infection in P. japonicus.

Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus. Publishing Authors By Initials

H Yang,T Luo,F Li,S Li,X Xu,

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Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Fish & shellfish immunology

VOLUME: 22

Page Numbers: 88-97

Journal Abbreviation: Fish Shellfish Immunol.

ISSN: 1050-4648

DAY: 1

MONTH: 04

YEAR: 2006

Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Purification and characterisation of a calcium-independent lectin (PjLec) from the haemolymph of the shrimp Penaeus japonicus.

AFFILIATION: College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, PR China. hjyang_wmd@hotmail.com

Country: England

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MEDLINETA: Fish Shellfish Immunol

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