Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom.

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Abstract Text:

S Y Liu,K Yoshizumi,N Oda,M Ohno,F Tokunaga,S Iwanaga,H Kihara,

A basic protein (pI 10.3), named basic protein II, was purified to homogeneity from the venom of Trimeresurus flavoviridis (Habu snake) after four chromatographic steps. The amino acid sequence of this protein was determined by sequencing the S-pyridylethylated derivative and its peptides produced by chemical (cyanogen bromide) and enzymatic (chymotrypsin, clostripain, and Staphylococcus aureus V8 protease) cleavages. The protein consisted of 122 amino acid residues and was found to be identical in sequence to basic protein I from the same source except that Asp-58 of basic protein I is replaced by asparagine. Like basic protein I, the structural feature of basic protein II is that Tyr-28 and Asp-49 common in phospholipases A2 from snake venoms and mammalian pancreas are replaced by asparagine and lysine, respectively. Thus, basic protein II belongs to the category of lysine-49-phospholipase A2. The action of basic protein II on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphorylcholine released only oleic acid, indicating that it has phospholipase A2 activity. Its molar activity toward 1,2-dilauroyl-sn-glycero-3-phosphorylcholine, however, was only 1.7% of that of T. flavoviridis phospholipase A2 isolated previously. Affinity for Ca2+ and reactivity toward p-bromophenacyl bromide of basic protein II were 8 and 5.3 times, respectively, smaller than those of phospholipase A2 from the same source, substantiating the low phospholipase A2 activity of basic protein II.

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Publishing Authors By Initials

SY Liu,K Yoshizumi,N Oda,M Ohno,F Tokunaga,S Iwanaga,H Kihara,

For similar proteins research abstracts see: proteins research

PUBMED ID PMID:

MEDLINE DATE:

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 107

Page Numbers: 400-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1990

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom. Information

Substance Name: Phospholipases A2

Registry Number: EC 3.1.1.4

Grant and Affiliation Information for Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom.

AFFILIATION: Department of Chemistry, Kyushu University, Fukuoka.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom Related Publications

• Apical cell membranes are shed into urine from injured podocytes in IgA nephropathy: Proposal of hypothesis about podocyte injury.
• Beta-alanine, like glycine, microinjected into the rat nucleus tractus solitarii increases blood pressure.
• Cloning and Characterization of the BLR2, the Homologue of the Blue-Light Regulator of Neurospora crassa WC-2, in the Phytopathogenic Fungus Bipolaris oryzae.
• Cloning and sequence analysis of cDNA for Trimeresurus flavoviridis phospholipase A2, and consequent revision of the amino acid sequence.
• Collecting duct carcinoma with acquired cystic disease of the kidney in a long-term hemodialysis patient.
• Comparative genomics of small RNAs in bacterial genomes.
• Development of synchrotron radiation storage rings.
• Dissociation and auto-oxidation of hemerythrin induced by SH-modification: a kinetic study.
• Dissociation of Limulus polyphemus (horseshoe crab) hemocyanin I. Solution X-ray scattering study in equilibrium.
• Function prediction of uncharacterized proteins.
• High-resolution X-ray topographic images of dislocations in a silicon crystal recorded using an X-ray zooming tube.
• Imaging soft X-ray microscope at Rits Synchrotron Radiation Center.
• Impact of newly diagnosed abnormal glucose tolerance on long-term prognosis in patients with acute myocardial infarction.
• Improved accuracy in predicting the presence of Gleason pattern 4/5 prostate cancer by three-dimensional 26-core systematic biopsy.
• Interaction of Trimeresurus flavoviridis phospholipase A2 and its fragment with calcium ion.
• Lymphovascular invasion is independently associated with poor prognosis in patients with localized upper urinary tract urothelial carcinoma treated surgically.
• Phase unwrapping method for three-dimensional stress analysis by scattered-light photoelasticity with unpolarized light. 2. Experiment.
• Properties of a DNA-binding protein in a culture medium of thymus cells.
• Protective effects of heme oxygenase-1 against cyclophosphamide-induced haemorrhagic cystitis in rats.
• Purification and amino acid sequence of basic protein II, a lysine-49-phospholipase A2 with low activity, from Trimeresurus flavoviridis venom.
• Purification and some properties of human liver iduronate sulfatase.
• Sequential bilateral minimum incision endoscopic radical nephrectomy in dialysis patients with bilateral renal cell carcinomas.
• Sexual behaviors and their correlates among young people in Mauritius: a cross-sectional study.
• Stopped-Flow Apparatus for X-ray Scattering and XAFS.
• Studies on intracellular structures of COS cells by X-ray microscopy.
• Studies on phospholipase A in Trimeresurus flaoviridis venom. III. Purification and some properties of phospholipase A inhibitor in Habu serum.
• Theoretical analysis of fluorescence light shifts in optically pumped cesium beam frequency standards.
• Unintended pregnancy and its risk factors among university students in eastern China.
• [Basis of a patient-nurse relationship formation]
• [Usefulness of 18F-fluorodeoxyglucose positron emission tomography/computerized tomography in diagnosis and treatment of pelvic recurrence of bladder cancer after radical cystectomy: a case report]