Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III.

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Abstract Text:

Takeshi Miyatsuka,Taka-aki Matsuoka,Toshihiko Shiraiwa,Tsunehiko Yamamoto,Itaru Kojima,Hideaki Kaneto,Takeshi Miyatsuka,Taka-aki Matsuoka,Toshihiko Shiraiwa,Tsunehiko Yamamoto,Itaru Kojima,Hideaki Kaneto,

Pancreatic and duodenal homeobox factor 1 (Pdx1) has been demonstrated to play a crucial role in pancreas development and in maintenance of mature beta-cell function. However, it remains to be elucidated how Pdx1 gene expression is regulated in non-beta cells during pancreas development. Pdx1 and Ptf1a are expressed in pancreatic progenitor cells, which give rise to all three types of pancreatic tissue. In addition, Ptf1a has been shown to bind the mammalian Suppressor of Hairless (RBP-J) within the PTF1 complex. Furthermore, loss-of-function approaches have revealed that all three factors are essential for early pancreas development. We therefore hypothesized that Ptf1a and RBP-J regulate expression of the Pdx1 gene in pancreatic precursors. Reporter gene analyses showed that Ptf1a transactivated Pdx1 promoter in pancreatic Panc-1 cells, which was enhanced by RBP-J. Deletion/mutation analyses of the Pdx1 promoter and electrophoretic gel-mobility shift assays identified the Ptf1a binding site in the well-conserved regulatory sequence domain, termed Area III, which was also confirmed by the chromatin immunoprecipitation assay. Furthermore, adenovirus-mediated overexpression of Ptf1a, together with RBP-J, markedly increased Pdx1 protein levels in pancreatic AR42J-B13 cells. Our data suggest a novel transcriptional network, where Ptf1a and RBP-J cooperatively regulate Pdx1 gene expression through binding to Area III.

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Publishing Authors By Initials

T Miyatsuka,TA Matsuoka,T Shiraiwa,T Yamamoto,I Kojima,H Kaneto,T Miyatsuka,TA Matsuoka,T Shiraiwa,T Yamamoto,I Kojima,H Kaneto,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 362

Page Numbers: 905-9

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 24

MONTH: 08

YEAR: 2007

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372516

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III.

AFFILIATION: Department of Internal Medicine and Therapeutics (A8), Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. miyatuka@medone.med.osaka-u.ac.jp

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biochem Biophys Res Commun

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III Related Publications

• Polypyrrole nanotube array sensor for enhanced adsorption of glucose oxidase in glucose biosensors.
• Ptf1a and RBP-J cooperate in activating Pdx1 gene expression through binding to Area III.
• Tissue-specific deletion of c-Jun in the pancreas has limited effects on pancreas formation.
• Transcription factors as therapeutic targets for diabetes.