PTEN and NDUFB8 aberrations in cervical cancer tissue.

PTEN and NDUFB8 aberrations in cervical cancer tissue. Research Abstract Details 

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PTEN and NDUFB8 aberrations in cervical cancer tissue. Abstract Text:

S M Hsieh,D J Maguire,N A Lintell,M McCabe,L R Griffiths,S M Hsieh,D J Maguire,N A Lintell,M McCabe,L R Griffiths,

Cervical cancer is one of the world's major health issues. Despite many studies in this field, the carcinogenetic events of malignant conversion in cervical tumours have not been significantly characterised. The first aim of this project was to investigate the mutation status of the tumour suppressor gene- Phosphatase and Tension Homolog (PTEN)--in cervical cancer tissue. The second aim of this study was the analysis in the same cervical cancer tissue for aberrations in the mitochondrial electron transport chain subunit gene NDUFB8, which is localised to the same chromosomal contig as PTEN. The third aim was the evaluation of the potential therapeutic anti-cancer drug 2,4-Thiazolidinediones (TZDs) and its affect in regulating the PTEN protein in a cervical cancer cell line (HeLa). To approach the aims, paraffin-embedded cancerous cervical tissue and non-cancerous cervical tissue were obtained. DNA recovered from those tissues was then used to investigate the putative genomic changes regarding the NDUFB8 gene utilising SYBR Green I Real-Time PCR. The PTEN gene was studied via Dual-Labelled probe Real-Time PCR. To investigate the protein expression change of the PTEN protein, HeLa cells were firstly treated with different concentrations of 2,4-Thiazolidinediones and the level of PTEN protein expression was then observed utilising standard protein assays. Results indicated that there were putative copy-number changes between the cancerous cervical tissue and non-cancerous cervical tissue, with regard to the PTEN locus. This implies a potential gain of the PTEN gene in cancerous cervical tissue. With regards to normal cervical tissue versus cancerous cervical tissue no significant melting temperature differences were observed with the SYBR Green I Real-Time PCR in respect to the NDUFB8 gene. A putative up-regulation of PTEN protein was observed in TZD treated HeLa cells.

PTEN and NDUFB8 aberrations in cervical cancer tissue. Publishing Authors By Initials

SM Hsieh,DJ Maguire,NA Lintell,M McCabe,LR Griffiths,SM Hsieh,DJ Maguire,NA Lintell,M McCabe,LR Griffiths,

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PTEN and NDUFB8 aberrations in cervical cancer tissue. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Advances in experimental medicine and biology

VOLUME: 599

Page Numbers: 31-6

Journal Abbreviation: Adv. Exp. Med. Biol.

ISSN: 0065-2598

DAY: 30

MONTH: 08

YEAR: 2007

PTEN and NDUFB8 aberrations in cervical cancer tissue. Information

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LANGUAGE: eng

NlmUniqueID: 121103

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Grant and Affiliation Information for PTEN and NDUFB8 aberrations in cervical cancer tissue.

AFFILIATION: School of Biomolecular and Biomedical Science, Griffith University, Queensland 4111, Australia.

Country: United States

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MEDLINETA: Adv Exp Med Biol

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