Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane.

Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Research Abstract Details 

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Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Abstract Text:

Arlinet Kierbel,Ama Gassama-Diagne,Claudia Rocha,Lilliana Radoshevich,Joan Olson,Keith Mostov,Joanne Engel,

Pseudomonas aeruginosa, an important human pathogen, preferentially binds and enters injured cells from the basolateral (BL) surface. We previously demonstrated that activation of phosphatidylinositol 3-kinase (PI3K) and Akt are necessary and sufficient for P. aeruginosa entry from the apical (AP) surface and that AP addition of phosphatidylinositol 3,4,5-trisphosphate (PIP3) is sufficient to convert AP into BL membrane (Kierbel, A., A. Gassama-Diagne, K. Mostov, and J.N. Engel. 2005. Mol. Biol. Cell. 16:2577-2585; Gassama-Diagne, A., W. Yu, M. ter Beest, F. Martin-Belmonte, A. Kierbel, J. Engel, and K. Mostov. 2006. Nat. Cell Biol. 8:963-970). We now show that P. aeruginosa subverts this pathway to gain entry from the AP surface. In polarized monolayers, P. aeruginosa binds near cell-cell junctions without compromising them where it activates and recruits PI3K to the AP surface. Membrane protrusions enriched for PIP3 and actin accumulate at the AP surface at the site of bacterial binding. These protrusions lack AP membrane markers and are comprised of BL membrane constituents, which are trafficked there by transcytosis. The end result is that this bacterium transforms AP into BL membrane, creating a local microenvironment that facilitates its colonization and entry into the mucosal barrier.

Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Publishing Authors By Initials

A Kierbel,A Gassama-Diagne,C Rocha,L Radoshevich,J Olson,K Mostov,J Engel,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: pseudomonadaceae: pseudomonas: pseudomonas aeruginosa research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: pseudomonadaceae: pseudomonas: pseudomonas aeruginosa research

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Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of cell biology

VOLUME: 177

Page Numbers: 21-7

Journal Abbreviation:

ISSN: 0021-9525

DAY: 2

MONTH: 04

YEAR: 2007

Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Keywords Mesh Terms:

KEYWORDS: Pseudomonas aeruginosa

MESH TERMS: pathogenicity

Chemical & Substance for Abstract: Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. Information

Substance Name: 1-Phosphatidylinositol 3-Kinase

Registry Number: EC 2.7.1.137

Grant and Affiliation Information for Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane.

AFFILIATION: Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL55980

ACRONYM: HL

MEDLINETA: J Cell Biol

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