Pseudo internal standard approach for label-free quantitative proteomics.

Pseudo internal standard approach for label-free quantitative proteomics. Research Abstract Details 

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Pseudo internal standard approach for label-free quantitative proteomics. Abstract Text:

Tsuyoshi Tabata,Toshitaka Sato,Junro Kuromitsu,Yoshiya Oda,Tsuyoshi Tabata,Toshitaka Sato,Junro Kuromitsu,Yoshiya Oda,Tsuyoshi Tabata,Toshitaka Sato,Junro Kuromitsu,Yoshiya Oda,

Quantitative proteome analysis has become a versatile tool to understand biological functions. Although stable isotope labeling is the most reliable method for quantitative mass spectrometry, preparation of isotope-labeled compounds is time-consuming and expensive. Simple label-free approaches have been introduced, but intensity-based quantitation without standards is not generally accepted as reliable, especially for small molecules. We have developed a novel label-free quantitative proteome analysis using pseudo internal standards (PISs). This idea was derived from northern blotting analysis, in which housekeeping genes are used as standards to normalize and compare target gene expression levels in different samples. In many proteomics studies, most proteins do not change their expression levels under different conditions, and therefore, these proteins can be employed as pseudo internal standards. This new approach is simple and does not require additional standards or labeling reagents. The PIS method represents a novel approach for mass spectrometry-based comprehensive quantitatitation and may also be applicable to quantitative metabolome analysis.

Pseudo internal standard approach for label-free quantitative proteomics. Publishing Authors By Initials

T Tabata,T Sato,J Kuromitsu,Y Oda,T Tabata,T Sato,J Kuromitsu,Y Oda,T Tabata,T Sato,J Kuromitsu,Y Oda,

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Pseudo internal standard approach for label-free quantitative proteomics. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 8440-5

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ISSN: 0003-2700

DAY: 12

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Eisai Co., Ltd. Laboratory of Core Technology, Tokodai 5-1-3, Tsukuba, Ibaraki 300-2635, Japan, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Saitama 332-0012, Japan.

Country: United States

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MEDLINETA: Anal Chem

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