Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline.

Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Research Abstract Details 

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Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Abstract Text:

Y Nishina,K Sato,K Shiga,

Trigonelline, i.e., N-methylnicotinate, which has a zwitterionic structure similar to a substrate D-amino acid, is a useful active site probe for D-amino acid oxidase (DAO). The affinity of trigonelline for DAO in the deprotonated state at the enzyme bound FAD 3-imino group is higher than in the neutral state, contrary to in the case of benzoate, which is a competitive inhibitor and is in a monoanionic form. The time course of the absorbance change was monitored for the binding of DAO with trigonelline by means of a stopped-flow technique. The reaction, on monitoring at 507 nm, was found to be biphasic at pH 8.3, with fast and slow phases. The dissociation of the 3-imino proton of the enzyme bound FAD was observed in the same time course as the slow phase. These results suggest that the positive charge of trigonelline exists near the 3-imino group of the enzyme bound FAD and interacts repulsively with the proton of the 3-imino group. The absorption spectra of the DAO-trigonelline complex at various pHs also support this hypothesis. In the catalysis of DAO, a similar mechanism may be involved, that is, the positive charge of a D-amino acid may interact repulsively with the 3-imino proton of the enzyme bound FAD, and this interaction may be important for the catalysis.

Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Publishing Authors By Initials

Y Nishina,K Sato,K Shiga,

For similar animals: chordata: vertebrates: mammals: artiodactyla: swine research abstracts see: animals: chordata: vertebrates: mammals: artiodactyla: swine research

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Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 107

Page Numbers: 726-31

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1990

Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Keywords Mesh Terms:

KEYWORDS: Swine

MESH TERMS: enzymology

Chemical & Substance for Abstract: Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline. Information

Substance Name: D-Amino-Acid Oxidase

Registry Number: EC 1.4.3.3

Grant and Affiliation Information for Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand, trigonelline.

AFFILIATION: Department of Physiology, Kumamoto University Medical School.

Country: JAPAN

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MEDLINETA: J Biochem

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