Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata.

Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Research Abstract Details 

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Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Abstract Text:

T Endo,M Oya,N Tamiya,T Miyazawa,

The molecular properties of phospholipases (PLases) A2 I and A2 III from a sea snake, Laticauda semifasciata, have been characterized by gel-filtration, as well as proton NMR, CD, UV absorption, and fluorescence spectroscopic methods. PLase A2 I exists as a monomer in aqueous solution in the presence or in the absence of Ca2+. The dissociation constants of the Ca2+-enzyme complexes have been determined for the two enzymes. The 270-mHz proton NMR spectra of PLases A2 I and A2 III have been measured, and the aromatic proton resonances of His-21 and His-48 in the active site have been assigned. By analyzing the pH dependence of the chemical shifts of the histidine proton resonances, pKa values have been determined for His-21 and His-48 with and without Ca2+. The conformational transitions have been found to take place at low pH or at high temperature (at approximately 65 degrees C). Fluorescence change of PLase A2 I upon addition of substrate analogs suggests that Trp-70 in PLase A2 I is involved in the binding to micellar substrates. The lack of Trp-70 in PLase A2 III is probably related to the low enzymatic activity as compared with that of PLase A2 I.

Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Publishing Authors By Initials

T Endo,M Oya,N Tamiya,T Miyazawa,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 101

Page Numbers: 795-804

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1987

Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Keywords Mesh Terms:

KEYWORDS: Temperature

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Chemical & Substance for Abstract: Proton nuclear magnetic resonance characterization of phospholipase A2 from Laticauda semifasciata. Information

Substance Name: Phospholipases A2

Registry Number: EC 3.1.1.4

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Country: JAPAN

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MEDLINETA: J Biochem

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