Protocols for three-dimensional molecular imaging using mass spectrometry.

Protocols for three-dimensional molecular imaging using mass spectrometry. Research Abstract Details 

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Protocols for three-dimensional molecular imaging using mass spectrometry. Abstract Text:

Andreas Wucher,Juan Cheng,Nicholas Winograd,

A protocol for three-dimensional molecular thin-film analysis is described that utilizes imaging time-of-flight secondary ion mass spectrometry and large-area atomic force microscopy. As a test study, a 300-nm trehalose film deposited on a Si substrate was structured by bombardment with a focused 15-keV Ga+ ion beam and analyzed using a 40-keV C60+ cluster ion beam. A three-dimensional sputter depth profile was acquired as a series of high-resolution lateral SIMS images with intermittent erosion cycles. As the most important result of this study, we find that the structured film exhibits a highly nonuniform erosion rate, thus preventing a simple conversion of primary ion fluence into eroded depth. Instead, the depth scale calibration must be performed individually on each pixel of the imaged area. The resulting laterally resolved depth profiles are discussed in terms of the chemical damage induced by the Ga+ bombardment along with the physics of the C60+ induced erosion process.

Protocols for three-dimensional molecular imaging using mass spectrometry. Publishing Authors By Initials

A Wucher,J Cheng,N Winograd,

For similar trehalose research abstracts see: trehalose research

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Protocols for three-dimensional molecular imaging using mass spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 5529-39

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 21

MONTH: 06

YEAR: 2007

Protocols for three-dimensional molecular imaging using mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Protocols for three-dimensional molecular imaging using mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Trehalose

MESH TERMS: chemistry

Chemical & Substance for Abstract: Protocols for three-dimensional molecular imaging using mass spectrometry. Information

Substance Name: fullerene C60

Registry Number: 99685-96-8

Grant and Affiliation Information for Protocols for three-dimensional molecular imaging using mass spectrometry.

AFFILIATION: Physics Department, University of Duisburg-Essen, 47048 Duisburg, Germany.

Country: United States

AGENCY: United States NIBIB

GRANT: EB002016-13

ACRONYM: EB

MEDLINETA: Anal Chem

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