Protocol for the fast chromatin immunoprecipitation (ChIP) method.

Protocol for the fast chromatin immunoprecipitation (ChIP) method. Research Abstract Details 

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Protocol for the fast chromatin immunoprecipitation (ChIP) method. Abstract Text:

Joel D Nelson,Oleg Denisenko,Karol Bomsztyk,Joel D Nelson,Oleg Denisenko,Karol Bomsztyk,

Chromatin and transcriptional processes are among the most intensively studied fields of biology today. The introduction of chromatin immunoprecipitations (ChIP) represents a major advancement in this area. This powerful method allows researchers to probe specific protein-DNA interactions in vivo and to estimate the density of proteins at specific sites genome-wide. We have introduced several improvements to the traditional ChIP assay, which simplify the procedure, greatly reducing the time and labor required to complete the assay. The simplicity of the method yields highly reproducible results. Our improvements facilitate the probing of multiple proteins in a single experiment, which allows for the simultaneous monitoring of many genomic events. This method is particularly useful in kinetic studies where multiple samples are processed at the same time. Starting with sheared chromatin, PCR-ready DNA can be isolated from 16-24 ChIP samples in 4-6 h using the fast method.

Protocol for the fast chromatin immunoprecipitation (ChIP) method. Publishing Authors By Initials

JD Nelson,O Denisenko,K Bomsztyk,JD Nelson,O Denisenko,K Bomsztyk,

For similar proteins: dna-binding proteins research abstracts see: proteins: dna-binding proteins research

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Protocol for the fast chromatin immunoprecipitation (ChIP) method. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 1

Page Numbers: 179-85

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2006

Protocol for the fast chromatin immunoprecipitation (ChIP) method. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

Protocol for the fast chromatin immunoprecipitation (ChIP) method. Keywords Mesh Terms:

KEYWORDS: DNA-Binding Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Protocol for the fast chromatin immunoprecipitation (ChIP) method. Information

Substance Name: DNA-Binding Proteins

Registry Number: 0

Grant and Affiliation Information for Protocol for the fast chromatin immunoprecipitation (ChIP) method.

AFFILIATION: Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98109, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM45134

ACRONYM: GM

MEDLINETA: Nat Protoc

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