Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells.

Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Research Abstract Details 

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Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Abstract Text:

Bradley R Kelemen,Mai Du,Rasmus B Jensen,

Proteorhodopsin is a family of over 50 proteins that provide phototrophic capability to marine bacteria by acting as light-powered proton pumps. The potential importance of proteorhodopsin to global ocean ecosystems and the possible applications of proteorhodopsin in optical data storage and optical signal processing have spurred diverse research in this new family of proteins. We show that proteorhodopsin expressed in Escherichia coli is functional and properly inserted in the membrane. At high expression levels, it appears to self-associate. We present a method for determining spectral properties of proteorhodopsin in intact E. coli cells that matches results obtained with detergent-solubilized, purified proteins. Using this method, we observe distinctly different spectra for protonated and deprotonated forms of 21 natural proteorhodopsin proteins in intact E. coli cells. Upon protonation, the wavelength maxima red shifts between 13 and 53 nm. We find that pKa values between 7.1 and 8.5 describe the pH-dependent spectral shift for all of the 21 natural variants of proteorhodopsin. The wavelength maxima of the deprotonated forms of the 21 natural proteorhodopsins cluster in two sequence-related groups: blue proteorhodopsins (B-PR) and green proteorhodopsins (G-PR). The site-directed substitution Leu105Gln in Bac31A8 proteorhodopsin shifts this G-PR's wavelength maximum to a wavelength maximum the same as that of the B-PR Hot75m1 proteorhodopsin. The site-directed substitution Gln107Leu in Hot75m1 proteorhodopsin shifts this B-PR's wavelength maximum to a wavelength maximum as that of Bac31A8 proteorhodopsin.

Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Publishing Authors By Initials

BR Kelemen,M Du,RB Jensen,

For similar genetic phenomena: variation (genetics) research abstracts see: genetic phenomena: variation (genetics) research

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Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 1618

Page Numbers: 25-32

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 3

MONTH: Dec

YEAR: 2003

Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Information

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LANGUAGE: eng

NlmUniqueID: 217513

Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Keywords Mesh Terms:

KEYWORDS: Variation (Genetics)

MESH TERMS: genetics

Chemical & Substance for Abstract: Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells. Information

Substance Name: Rhodopsin

Registry Number: 9009-81-8

Grant and Affiliation Information for Proteorhodopsin in living color: diversity of spectral properties within living bacterial cells.

AFFILIATION: Genencor International, Inc, 925 Page Mill Road, Palo Alto, CA 94304, USA.

Country: Netherlands

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MEDLINETA: Biochim Biophys Acta

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