Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein.

Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Research Abstract Details 

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Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Abstract Text:

Shigeko Sato,Masayoshi Fukasawa,Yoshio Yamakawa,Tohru Natsume,Tetsuro Suzuki,Ikuo Shoji,Hideki Aizaki,Tatsuo Miyamura,Masahiro Nishijima,

Hepatitis C virus (HCV) core protein has been suggested to play crucial roles in the pathogeneses of liver steatosis and hepatocellular carcinomas due to HCV infection. Intracellular HCV core protein is localized mainly in lipid droplets, in which the core protein should exert its significant biological/pathological functions. In this study, we performed comparative proteomic analysis of lipid droplet proteins in core-expressing and non-expressing hepatoma cell lines. We identified 38 proteins in the lipid droplet fraction of core-expressing (Hep39) cells and 30 proteins in that of non-expressing (Hepswx) cells by 1-D-SDS-PAGE/MALDI-TOF mass spectrometry (MS) or direct nanoflow liquid chromatography-MS/MS. Interestingly, the lipid droplet fraction of Hep39 cells had an apparently lower content of adipose differentiation-related protein and a much higher content of TIP47 than that of Hepswx cells, suggesting the participation of the core protein in lipid droplet biogenesis in HCV-infected cells. Another distinct feature is that proteins involved in RNA metabolism, particularly DEAD box protein 1 and DEAD box protein 3, were detected in the lipid droplet fraction of Hep39 cells. These results suggest that lipid droplets containing HCV core protein may participate in the RNA metabolism of the host and/or HCV, affecting the pathopoiesis and/or virus replication/production in HCV-infected cells.

Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Publishing Authors By Initials

S Sato,M Fukasawa,Y Yamakawa,T Natsume,T Suzuki,I Shoji,H Aizaki,T Miyamura,M Nishijima,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 921-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2006

Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. Information

Substance Name: DDX3X protein, human

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein.

AFFILIATION: Department of Biochemistry and Cell Biology and Department of Virology II, National Institute of Infectious Diseases, Tokyo 162-8640.

Country: Japan

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MEDLINETA: J Biochem

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