Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol.

Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol. Research Abstract Details 

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Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol. Abstract Text:

Chinese hamster ovary (CHO) cells are widely used for the production of recombinant protein biopharmaceuticals. The purpose of this study was to investigate differences in the proteome of CHO DUKX cells expressing recombinant human bone morphogenetic protein-2 (rhBMP-2) (G5 cells) compared to cells also expressing soluble exogenous paired basic amino acid cleaving enzyme soluble paired basic amino acid cleaving enzyme (PACEsol) (3C9 cells), which has been previously found to improve the post-translational processing of the mature rhBMP-2 dimer. PACEsol co-expression was also associated with a significant increase (almost four-fold) in cellular productivity of rhBMP-2 protein. Differential proteomic expression profiling using 2-D DIGE and MALDI-TOF MS was performed to compare 3C9 and G5 cells, and revealed a list of 60 proteins that showed differential expression (up/downregulated), with a variety of different cellular functions. A substantial number of these altered proteins were found to have chaperone activity, involved with protein folding, assembly and secretion, as well as a number of proteins involved in protein translation. These results support the use of proteomic profiling as a valuable tool towards understanding the biology of bioprocess cultures.

Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol. Publishing Authors By Initials

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Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 8

Page Numbers: 2611-24

Journal Abbreviation: Proteomics

ISSN: 1615-9861

DAY: 17

MONTH: Jul

YEAR: 2008

Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol. Information

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LANGUAGE: eng

NlmUniqueID: 101092707

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Grant and Affiliation Information for Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol.

AFFILIATION: National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland. paula.meleady@dcu.ie

Country: Germany

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MEDLINETA: Proteomics

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