Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer.

Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer. Research Abstract Details 

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Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer. Abstract Text:

David Jackson,Rachel A Craven,Richard C Hutson,Ina Graze,Paul Lueth,Robert P Tonge,Joanne L Hartley,Janice A Nickson,Steve J Rayner,Colin Johnston,Benjamin Dieplinger,Michael Hubalek,Nafisa Wilkinson,Timothy J Perren,Sean Kehoe,Geoffrey D Hall,Guenter Daxenbichler,Hans Dieplinger,Peter J Selby,Rosamonde E Banks,David Jackson,Rachel A Craven,Richard C Hutson,Ina Graze,Paul Lueth,Robert P Tonge,Joanne L Hartley,Janice A Nickson,Steve J Rayner,Colin Johnston,Benjamin Dieplinger,Michael Hubalek,Nafisa Wilkinson,Timothy J Perren,Sean Kehoe,Geoffrey D Hall,Guenter Daxenbichler,Hans Dieplinger,Peter J Selby,Rosamonde E Banks,

PURPOSE: To discover and validate serum glycoprotein biomarkers in ovarian cancer using proteomic-based approaches. EXPERIMENTAL DESIGN: Serum samples from a "discovery set" of 20 patients with ovarian cancer or benign ovarian cysts or healthy volunteers were compared by fluorescence two-dimensional differential in-gel electrophoresis and parallel lectin-based two-dimensional profiling. Validation of a candidate biomarker was carried out with Western blotting and immunoassay (n = 424). RESULTS: Twenty-six proteins that changed significantly were identified by mass spectrometric sequencing. One of these, confirmed by Western blotting, was afamin, a vitamin E binding protein, with two isoforms decreasing in patients with ovarian cancer. Validation using cross-sectional samples from 303 individuals (healthy controls and patients with benign, borderline, or malignant ovarian conditions and other cancers) assayed by ELISA showed significantly decreased total afamin concentrations in patients with ovarian cancer compared with healthy controls (P = 0.002) and patients with benign disease (P = 0.046). However, the receiver operating characteristic areas for total afamin for the comparison of ovarian cancer with healthy controls or benign controls were only 0.67 and 0.60, respectively, with comparable figures for CA-125 being 0.92 and 0.88 although corresponding figures for a subgroup of samples analyzed by isoelectric focusing for afamin isoform 2 were 0.85 and 0.79. Analysis of a further 121 samples collected prospectively from 9 patients pretreatment through to relapse indicated complementarity of afamin with CA-125, including two cases in whom CA-125 was noninformative. CONCLUSIONS: Afamin shows potential complementarity with CA-125 in longitudinal monitoring of patients with ovarian cancer, justifying prospective larger-scale investigation. Changes in specific isoforms may provide further information.

Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer. Publishing Authors By Initials

D Jackson,RA Craven,RC Hutson,I Graze,P Lueth,RP Tonge,JL Hartley,JA Nickson,SJ Rayner,C Johnston,B Dieplinger,M Hubalek,N Wilkinson,TJ Perren,S Kehoe,GD Hall,G Daxenbichler,H Dieplinger,PJ Selby,RE Banks,D Jackson,RA Craven,RC Hutson,I Graze,P Lueth,RP Tonge,JL Hartley,JA Nickson,SJ Rayner,C Johnston,B Dieplinger,M Hubalek,N Wilkinson,TJ Perren,S Kehoe,GD Hall,G Daxenbichler,H Dieplinger,PJ Selby,RE Banks,

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Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical cancer research : an official journal of

VOLUME: 13

Page Numbers: 7370-9

Journal Abbreviation: Clin. Cancer Res.

ISSN: 1078-0432

DAY: 15

MONTH: Dec

YEAR: 2007

Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer. Information

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LANGUAGE: eng

NlmUniqueID: 9502500

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AFFILIATION: Authors' Affiliations: Cancer Research UK Clinical Centre, Department of Obstetrics and Gynaecology, and Department of Pathology, St James's University Hospital, Leeds, United Kingdom.

Country: United States

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MEDLINETA: Clin Cancer Res

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