Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit.

Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit. Research Abstract Details 

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Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit. Abstract Text:

Kazunobu Saito,Kengo Enya,Chitose Oneyama,Tomoya Hikita,Masato Okada,Kazunobu Saito,Kengo Enya,Chitose Oneyama,Tomoya Hikita,Masato Okada,

To elucidate the regulatory mechanism of cell transformation induced by c-Src tyrosine kinase, we performed a proteomic analysis of tyrosine phosphorylated proteins that interact with c-Src and/or its negative regulator Csk. The c-Src interacting proteins were affinity-purified from Src transformed cells using the Src SH2 domain as a ligand. LC-MS/MS analysis of the purified proteins identified general Src substrates, such as focal adhesion kinase and paxillin, and ZO-1/2 as a transformation-dependent Src target. The Csk binding proteins were analyzed by a tandem affinity purification method. In addition to the previously identified Csk binding proteins, including Cbp/PAG, paxillin, and caveolin-1, we found that ZO-1/2 could also serve as a major Csk binding protein. ZO-2 was phosphorylated concurrently with Src transformation and specifically bound to Csk in a Csk SH2 dependent manner. These results suggest novel roles for ZO proteins as Src/Csk scaffolds potentially involved in the regulation of Src transformation.

Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit. Publishing Authors By Initials

K Saito,K Enya,C Oneyama,T Hikita,M Okada,K Saito,K Enya,C Oneyama,T Hikita,M Okada,

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Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 366

Page Numbers: 969-75

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 18

MONTH: 12

YEAR: 2007

Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Proteomic identification of ZO-1/2 as a novel scaffold for Src/Csk regulatory circuit.

AFFILIATION: Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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