Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. Research Abstract Details 

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Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. Abstract Text:

Julia ,Lasse Weinmann,Christine Ender,Sabine ,Elisabeth Kremmer,Monika Raabe,Henning Urlaub,Gunter Meister,Julia ,Lasse Weinmann,Christine Ender,Sabine ,Elisabeth Kremmer,Monika Raabe,Henning Urlaub,Gunter Meister,

Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. Publishing Authors By Initials

J ,L Weinmann,C Ender,S ,E Kremmer,M Raabe,H Urlaub,G Meister,J ,L Weinmann,C Ender,S ,E Kremmer,M Raabe,H Urlaub,G Meister,

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Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: EMBO reports

VOLUME: 8

Page Numbers: 1052-60

Journal Abbreviation: EMBO Rep.

ISSN: 1469-221X

DAY: 12

MONTH: 10

YEAR: 2007

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. Information

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LANGUAGE: eng

NlmUniqueID: 100963049

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Grant and Affiliation Information for Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

AFFILIATION: Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

Country: England

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MEDLINETA: EMBO Rep

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