Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry.

Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Research Abstract Details 

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Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Abstract Text:

Ningjia He,Julianne M C Botelho,Rebecca J McNall,Vladimir Belozerov,W Augustine Dunn,Todd Mize,Ron Orlando,Judith H Willis,

Identification of authenticated cuticular proteins has been based on isolation and sequencing of individual proteins extracted from cleaned cuticles. These data facilitated classification of sequences from conceptual translation of cDNA or genomic sequences. The question arises whether such putative cuticular proteins actually are incorporated into the cuticle. This paper describes the profiling of cuticular proteins from Anopheles gambiae starting with cuticle cleaned by the insect itself in the course of molting. Proteins extracted from cast larval head capsules and cast pupal cuticles were fractionated by 1D SDS gel electrophoresis. Large gel slices were reduced, carbamidomethylated and digested with trypsin. The pellet remaining after SDS extraction was also treated with trypsin. The resulting peptides were separated on a C18 column and then analyzed by tandem mass spectrometry. Two-hundred-ninety-five peptides from putative cuticular proteins were identified; these corresponded to a minimum of 69 and a maximum of 119 different proteins. Each is reported as an authentic Anopheles cuticular protein for the first time. In addition to members of two known cuticular protein families, members of additional families likely to be structural components of the cuticle were identified. Furthermore, other peptides were identified that can be attributed to molting fluid, muscle and sclerotizing agents.

Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Publishing Authors By Initials

N He,JM Botelho,RJ McNall,V Belozerov,WA Dunn,T Mize,R Orlando,JH Willis,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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MEDLINE DATE:

Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Insect biochemistry and molecular biology

VOLUME: 37

Page Numbers: 135-46

Journal Abbreviation: Insect Biochem. Mol. Biol.

ISSN: 0965-1748

DAY: 14

MONTH: 11

YEAR: 2006

Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9207282

Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: methods

Chemical & Substance for Abstract: Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry. Information

Substance Name: cuticle proteins, insects

Registry Number: 0

Grant and Affiliation Information for Proteomic analysis of cast cuticles from Anopheles gambiae by tandem mass spectrometry.

AFFILIATION: The Key Laboratory of Sericulture, Southwest University, Chongqing 400715, PR China.

Country: England

AGENCY: United States NCRR

GRANT: P41RR018502

ACRONYM: RR

MEDLINETA: Insect Biochem Mol Biol

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