Proteome analysis of chemically induced mouse liver tumors with different genotype.

Proteome analysis of chemically induced mouse liver tumors with different genotype. Research Abstract Details 

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Proteome analysis of chemically induced mouse liver tumors with different genotype. Abstract Text:

Julia Strathmann,Krisztina Paal,Carina Ittrich,Eberhard Krause,Klaus E Appel,Howard P Glauert,Albrecht Buchmann,Michael Schwarz,

Mouse liver tumors frequently harbor mutations in Ha-ras, B-raf, or Ctnnb1 (encoding beta-catenin). We conducted a proteome analysis with protein extracts from normal mouse liver and from liver tumors which were induced by a single injection of N-nitrosodiethylamine (DEN) as initiator followed by multiple injections of two different polychlorinated biphenyls (PCBs) as tumor promoters, or corn oil as a control. Liver tumors were stratified into two classes: they were either mutated in Ctnnb1 and positive for the marker glutamine synthetase (GS(+)), or they lacked Ctnnb1 mutations and were therefore GS-negative (GS(-)). Proteome analysis by 2-DE and MS revealed 98 significantly deregulated proteins, 44 in GS(+) and 54 in GS(-) tumors. Twelve of these proteins showed expression changes in both tumor types, but only seven of them were deregulated in the same direction. Several of the identified enzymes could be assigned to fundamental metabolic or other cellular pathways with characteristically different alterations in GS(+) and GS(-) tumors such as ammonia and amino acid turnover, cellular energy supply, and calcium homeostasis. Our data suggest that GS(+) and GS(-) tumor cells show a completely different biology and use divergent evolutionary strategies to gain a selective advantage over normal hepatocytes.

Proteome analysis of chemically induced mouse liver tumors with different genotype. Publishing Authors By Initials

J Strathmann,K Paal,C Ittrich,E Krause,KE Appel,HP Glauert,A Buchmann,M Schwarz,

For similar proteins: proteome research abstracts see: proteins: proteome research

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Proteome analysis of chemically induced mouse liver tumors with different genotype. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 7

Page Numbers: 3318-31

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 3

MONTH: Sep

YEAR: 2007

Proteome analysis of chemically induced mouse liver tumors with different genotype. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101092707

Proteome analysis of chemically induced mouse liver tumors with different genotype. Keywords Mesh Terms:

KEYWORDS: Proteome

MESH TERMS: toxicity

Chemical & Substance for Abstract: Proteome analysis of chemically induced mouse liver tumors with different genotype. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Proteome analysis of chemically induced mouse liver tumors with different genotype.

AFFILIATION: Department of Toxicology, University of Tübingen, Tübingen, Germany.

Country: Germany

AGENCY: United States NIEHS

GRANT: ES-07380

ACRONYM: ES

MEDLINETA: Proteomics

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