Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers.

Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Research Abstract Details 

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Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Abstract Text:

Yifan Yang,Bozena Jemiolo,Scott Trappe,

The purpose of this study was to characterize changes in mRNA expression of select proteolytic markers in human slow-twitch [myosin heavy chain (MHC) I] and fast-twitch (MHC IIa) single skeletal muscle fibers following a bout of resistance exercise (RE). Muscle biopsies were obtained from the vastus lateralis of eight young healthy sedentary men [23 +/- 2 yr (mean +/- SD), 93 +/- 17 kg, 183 +/- 6 cm] before and 4 and 24 h after 3 x 10 repetitions of bilateral knee extensions at 65% of one repetition maximum. The mRNA levels of TNF-alpha, calpains 1 and 2, muscle RING (really interesting novel gene) finger-1 (MuRF-1), atrogin-1, caspase-3, B-cell leukemia/lymphoma (Bcl)-2, and Bcl-2-associated X protein (Bax) were quantified using real-time RT-PCR. Generally, MHC I fibers had higher (1.6- to 5.0-fold, P < 0.05) mRNA expression pre- and post-RE. One exception was a higher (1.6- to 3.9-fold, P < 0.05) Bax-to-Bcl-2 mRNA ratio in MHC IIa fibers pre- and post-RE. RE increased (1.4- to 4.8-fold, P < 0.05) MuRF-1 and caspase-3 mRNA levels 4-24 h post-RE in both fiber types, whereas Bax-to-Bcl-2 mRNA ratio increased 2.2-fold (P < 0.05) at 4 h post-RE only in MHC I fibers. These results suggest that MHC I fibers have a greater proteolytic mRNA expression pre- and post-RE compared with MHC IIa fibers. The greatest mRNA induction following RE was in MuRF-1 and caspase-3 in both fiber types. This altered and specific proteolytic mRNA expression among slow- and fast-twitch muscle fibers indicates that the ubiquitin/proteasomal and caspase pathways may play an important role in muscle remodeling with RE.

Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Publishing Authors By Initials

Y Yang,B Jemiolo,S Trappe,

For similar human activities: leisure activities: recreation: sports: weight lifting research abstracts see: human activities: leisure activities: recreation: sports: weight lifting research

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MEDLINE DATE:

Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of applied physiology (Bethesda, Md. : 198

VOLUME: 101

Page Numbers: 1442-50

Journal Abbreviation: J. Appl. Physiol.

ISSN: 8750-7587

DAY: 13

MONTH: 07

YEAR: 2006

Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8502536

Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Keywords Mesh Terms:

KEYWORDS: Weight Lifting

MESH TERMS: physiology

Chemical & Substance for Abstract: Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers.

AFFILIATION: Human Performance Laboratory, Ball State University, Muncie, IN 47306, USA.

Country: United States

AGENCY: United States NIA

GRANT: AG-18409

ACRONYM: AG

MEDLINETA: J Appl Physiol

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