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Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset.

Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset. Research Abstract Details 

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  • Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset. Abstract Text:

    During Escherichia coli growth on glucose, uptake exceeds the requirement of flux to precursors and the surplus is excreted as acetate. Beside the loss of carbon source, the excretion of a weak acid may result in increased energetic demands and hence a decreased yield. The deletion of ptsG, the gene coding for one of the components (IICB(Glc)) of the glucose-phosphoenolpyruvate phosphotransferase system (Glc-PTS) reduced glucose consumption and acetate excretion. Induction of protein production at the onset of cultivation decreased growth rate and glucose consumption rate for both the WT and the mutant strains. The mutant strain produced beta-galactosidase at higher rates than the wild-type strain while directing more carbon into biomass and CO(2) and less into acetate.

    Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset. Publishing Authors By Initials

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    PUBMED ID PMID:

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    Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of industrial microbiology & biotechnology

    VOLUME: 35

    Page Numbers: 213-8

    Journal Abbreviation: J. Ind. Microbiol. Biotechnol.

    ISSN: 1367-5435

    DAY: 8

    MONTH: 01

    YEAR: 2008

    Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset. Information

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    LANGUAGE: eng

    NlmUniqueID: 9705544

    Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset. Keywords Mesh Terms:

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    Grant and Affiliation Information for Protein production by Escherichia coli wild-type and DeltaptsG mutant strains with IPTG induction at the onset.

    AFFILIATION: SILS, BioCentrum Amsterdam, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, The Netherlands, apicon@inia.es.

    Country: England

    England Research PublicationEngland Research Publication

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    MEDLINETA: J Ind Microbiol Biotechnol

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