Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells.

Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Research Abstract Details 

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Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Abstract Text:

Virna L Sales,George C Engelmayr,John A Johnson,Jin Gao,Yadong Wang,Michael S Sacks,John E Mayer,

BACKGROUND: Optimal cell sources and scaffold-cell interactions remain unanswered questions for tissue engineering of heart valves. We assessed the effect of different protein precoatings on a single scaffold type (elastomeric poly (glycerol sebacate)) with a single cell source (endothelial progenitor cells). METHODS AND RESULTS: Elastomeric poly (glycerol sebacate) scaffolds were precoated with laminin, fibronectin, fibrin, collagen types I/III, or elastin. Characterized ovine peripheral blood endothelial progenitor cells were seeded onto scaffolds for 3 days followed by 14 days incubation. Endothelial progenitor cells were CD31+, vWF+, and alpha-SMA- before seeding confirmed by immunohistochemistry and immunoblotting. Both precoated and uncoated scaffolds demonstrated surface expression of CD31+ and vWF+, alpha-SMA+ cells and were found in the "interstitium" of the scaffold. Protein precoating of elastomeric poly (glycerol sebacate) scaffolds revealed significantly increased cellularity and altered the phenotypes of endothelial progenitor cells, which resulted in changes in cellular behavior and extracellular matrix production. Moreover, mechanical flexure testing demonstrated decreased effective stiffness of the seeded scaffolds compared with unseeded controls. CONCLUSIONS: Scaffold precoating with extracellular matrix proteins can allow more precise "engineering" of cellular behavior in the development of tissue engineering of heart valves constructs by altering extracellular matrix production and cell phenotype.

Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Publishing Authors By Initials

VL Sales,GC Engelmayr,JA Johnson,J Gao,Y Wang,MS Sacks,JE Mayer,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research

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Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Circulation

VOLUME: 116

Page Numbers: I55-63

Journal Abbreviation: Circulation

ISSN: 1524-4539

DAY: 11

MONTH: Sep

YEAR: 2007

Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Information

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LANGUAGE: eng

NlmUniqueID: 147763

Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Keywords Mesh Terms:

KEYWORDS: Tissue Engineering

MESH TERMS: methods

Chemical & Substance for Abstract: Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells. Information

Substance Name: elastomeric

Registry Number: 0

Grant and Affiliation Information for Protein precoating of elastomeric tissue-engineering scaffolds increased cellularity, enhanced extracellular matrix protein production, and differentially regulated the phenotypes of circulating endothelial progenitor cells.

AFFILIATION: Department of Cardiac Surgery, Children's Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA. virna.sales@cardio.chboston.org

Country: United States

AGENCY: United States NHLBI

GRANT: HL-68816-01

ACRONYM: HL

MEDLINETA: Circulation

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