Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins.

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Abstract Text:

H Qi,X Zheng,X Qin,D Dou,H Xu,J U Raj,Y Gao,H Qi,X Zheng,X Qin,D Dou,H Xu,J U Raj,Y Gao,H Qi,X Zheng,X Qin,D Dou,H Xu,J U Raj,Y Gao,

Background and purpose:Coronary venous activity is modulated by endogenous and exogenous nitrovasodilators. The present study was to determine the role of protein kinase G (PKG) in the regulation of the basal tension and nitrovasodilator-induced relaxation of coronary veins.Experimental approach:Effects of a PKG inhibitor on the basal tension and responses induced by nitroglycerin, DETA NONOate, and 8-Br-cGMP in isolated porcine coronary veins were determined. Cyclic cGMP was measured with radioimmunoassay. PKG activity was determined by measuring the incorporation of (32)P from gamma-(32)P-ATP into the specific substrate BPDEtide.Key results:Rp-8-Br-PET-cGMPS, a specific PKG inhibitor, increased the basal tension of porcine coronary veins and decreased PKG activity. The increase in tension was 38% of that caused by nitro-L-arginine. Relaxation of the veins induced by nitroglycerin and DETA NONOate was accompanied with increases in cGMP content and PKG activity. These effects were largely eliminated by inhibiting soluble guanylyl cyclase with ODQ. The increase in PKG activity induced by the nitrovasodilators was abolished by Rp-8-Br-PET-cGMPS. The relaxation caused by these dilators and by 8-Br-cGMP at their EC(50) was attenuated by the PKG inhibitor by 51-66%.Conclusions and implications:These results suggest that PKG is critically involved in nitric oxide-mediated regulation of the basal tension in porcine coronary veins and that it plays a primary role in relaxation induced by nitrovasodilators. Since nitric oxide plays a key role in modulating coronary venous activity, augmentation of PKG may be a therapeutic target for improving coronary blood flow.British Journal of Pharmacology (2007) 152, 1060-1069; doi:10.1038/sj.bjp.0707479; published online 24 September 2007.

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Publishing Authors By Initials

H Qi,X Zheng,X Qin,D Dou,H Xu,JU Raj,Y Gao,H Qi,X Zheng,X Qin,D Dou,H Xu,JU Raj,Y Gao,H Qi,X Zheng,X Qin,D Dou,H Xu,JU Raj,Y Gao,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: British journal of pharmacology

VOLUME: 152

Page Numbers: 1060-9

Journal Abbreviation: Br. J. Pharmacol.

ISSN: 0007-1188

DAY: 24

MONTH: 09

YEAR: 2007

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7502536

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins.

AFFILIATION: 1Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, China.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Br J Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins Related Publications

• Clinicopathologic significance of structural alterations of p53 protein in papillary thyroid carcinoma.
• Comparison of doxorubicin concentration profiles in radiofrequency-ablated rat livers from sustained- and dual-release PLGA millirods.
• Engineering temperature-sensitive poly(N-isopropylacrylamide) polymers as carriers of therapeutic proteins.
• Hath1, down-regulated in colon adenocarcinomas, inhibits proliferation and tumorigenesis of colon cancer cells.
• Optical design of the NSRL undulator beamline.
• Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression.
• [9-cis retinoic acid induces apoptosis in HL-60 cells]
• [A study of human annexin V derivative: its effects of anticoagulation and antithrombosis.]
• [Accurate diagnosis of stages of hepatic fibrosis by measuring levels of serum hyaluronic acid, procollagen type III, and collagen type IV]
• [Adenovirus-mediated transfer of TIMP-4 gene inhibits neointimal formation after balloon injury]
• [Analysis of 37 pathological and clinic data of extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT)]
• [Association of insertion/deletion polymorphism in angiotensin-converting enzyme gene with hypertensive type 2 diabetes mellitus]
• [Cloning and expression of Trichoderma reesei endoglucanase III (EG III) gene in Saccharomyces cerevasiae]
• [Comparison of severe acute respiratory syndrome with community-acquired pneumonia]
• [Constructing tandem-repeated sequence of nucleic acid and evaluating its signal amplification action]
• [Current status and distribution of deafness in the elderly in several cities in China]
• [Evaluation of the effect of intervened measures for health behavior of lead exposed workers]
• [Experimental study on a novel esophageal prosthesis made of composite biomaterials]
• [Expression of chemokine-like factor 2 (CKLF2) mRNA in the rat myocardium after myocardial infarction]
• [MMF and CyA in the prevention of early acute rejection after renal transplantation]
• [Optimizing the synthesis of hydroxyapatite as a method for the defluoridation of drinking water]
• [Protein C system and sepsis]
• [Purification and properties of endoglucanases from Aspergilius aculeatus SM-L22]
• [Redescription of Prorocentrum donghaiense Lu and comparison with relevant Prorocentrum species]
• [Removal of different species of red tide organisms with an effective clay-complex system]
• [Study on a new antarthritic injection--O-carboxymethyl chitosan]
• [Study on the targeting effects of M1-GS RNA on K562 cells.]
• [Surgical treatment of left main stenosis in coronary artery disease]
• [The construction of monoclonal antibody cell line against Schistosoma japonicum recombinant protein 20.8 kDa antigen]
• [The cryopreservation of human oocytes at different maturity stages.]