Protein kinase C activity in mouse eggs regulates gamete membrane interaction.

Protein kinase C activity in mouse eggs regulates gamete membrane interaction. Research Abstract Details 

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Protein kinase C activity in mouse eggs regulates gamete membrane interaction. Abstract Text:

Hiroto Akabane,Jun Fan,Xuehai Zheng,Guo-Zhang Zhu,Hiroto Akabane,Jun Fan,Xuehai Zheng,Guo-Zhang Zhu,

Gamete membrane interaction is critical to initiate the development of a new organism. The signaling pathways governing this event, however, are poorly understood. In this report, we provide the first evidence that protein kinase C activity in mouse eggs plays a crucial role in the regulation of this process. Stimulating PKC activity in mouse eggs by phorbol 12-myristate 13-acetate (PMA) drastically inhibited the egg's membrane ability to bind and fuse with sperm. Surprisingly, this significant reduction of gamete membrane interaction was also observed in eggs treated with the PKC inhibitors staurosporine and calphostin c. In further analysis, we found that while no change of egg actin cytoskeleton was detected after either PMA or calphostin c treatment, the structural morphology of egg surface microvilli was severely altered in the PMA-treated eggs, but not in the calphostin c-treated eggs. Moreover, sperm, which bound but did not fuse with the eggs treated with the anti-CD9 antibody KMC8, were liberated from the egg membrane after PMA, but not calphostin c, treatment. Taken together, these results suggest that egg PKC may be precisely balanced to regulate gamete membrane interaction in a biphasic mode, and this biphasic regulation is executed through two different mechanisms.

Protein kinase C activity in mouse eggs regulates gamete membrane interaction. Publishing Authors By Initials

H Akabane,J Fan,X Zheng,GZ Zhu,H Akabane,J Fan,X Zheng,GZ Zhu,

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Protein kinase C activity in mouse eggs regulates gamete membrane interaction. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular reproduction and development

VOLUME: 74

Page Numbers: 1465-72

Journal Abbreviation: Mol. Reprod. Dev.

ISSN: 1040-452X

DAY: 4

MONTH: Nov

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Protein kinase C activity in mouse eggs regulates gamete membrane interaction.

AFFILIATION: Department of Biological Sciences, Marshall University, Huntington, WV 25755, USA.

Country: United States

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MEDLINETA: Mol Reprod Dev

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