Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain.

Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain. Research Abstract Details 

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Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain. Abstract Text:

The chemical adsorption of organic phosphate compounds to alumina has been used to create surface linkers for protein immobilization. A number of particulate alumina supports were screened for their physical properties and ability to bind organic phosphate compounds. Two aluminas, termed C1 and CPC, were selected based on their suitability for subsequent testing as protein immobilization supports. Papain was successfully immobilized to these supports when derivatized with phosphate compounds containing free terminal carboxyl groups. Protein binding was enhanced when support carboxyl groups were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. The level of papain immobilization was dependent upon the length of the linker used and the mass of protein exposed to the support. (c) 1992 John Wiley & Sons, Inc.

Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain. Publishing Authors By Initials

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Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology and bioengineering

VOLUME: 40

Page Numbers: 1319-27

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 0006-3592

DAY: 20

MONTH: Dec

YEAR: 1992

Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Protein immobilization to alumina supports: I. Characterization of alumina-organophosphate ligand interactions and use in the attachment of papain.

AFFILIATION: Department of Biochemistry, Queen's University, Kingston, Canada.

Country: United States

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MEDLINETA: Biotechnol Bioeng

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